Schleifer-Krไmer Agar

(SK Agar)

Composition per liter:

Agar .............................................................. ...13.0g

Glycerol ........................................................ ...10.0g

Sodium pyruvate............................................. ..10.0g

Pancreatic digest of casein............................... ..10.0g

Beef extract...................................................... ...5.0g

Yeast extract...................................................... ..3.0g

Potassium isothiocyanate................................. .2.25g

LiCl.................................................................. ...2.0g

Na2HPO4*2H2O............................................... ...0.9g

NaH2PO4*H2O.................................................. ..0.6g

Glycine............................................................... .0.5g

NaN3 solution ............................................ .. 10.0mL

pH 7.2 ± 0.2 at 25oC

NaN3 Solution:

Composition per 10.0mL:

NaN3 ........................................................... ...0.045g

Preparation of NaN3 Solution: Add NaN3 to distilled/deionized  water  and  bring  volume  to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept NaN3 solution, to distilled/deionized water and
bring volume to 990.0mL. Mix thoroughly. Adjust
pH to 7.2. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure-121oC. Cool to
45o-50oC. Aseptically add sterile NaN3 solution.
Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.

Use: For the isolation and cultivation of Staphylo-
coccus species.


Selenite Broth

(Selenite Broth, Lactose)

(Selenite F Enrichment Medium)

(Sodium Biselenite Medium)
   (Sodium Hydrogen Selenite Medium)
Composition per liter:

Na2HPO4........................................................ ..10.0g

Pancreatic digest of casein.................................. .5.0g

Lactose............................................................... .4.0g

NaHSeO3*5H2O .............................................. ..4.0g

pH 7.0 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems and a prepared medium from Oxoid Unipath.

Caution: Sodium biselenite is toxic and a potential

teratogen and care must be taken to avoid inhalation of the powdered dye, contact with the skin, or inges-
tion, especially in pregnant laboratory workers.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling. Do
not autoclave. Distribute into sterile tubes in 10.0mL
volumes.

Use: For the isolation and enrichment of Salmonella species from clinical specimens.


Selenite Broth Base, Mannitol

Composition per liter:

Na2HPO4........................................................ ..10.0g

Peptone.............................................................. .5.0g

Mannitol........................................................... ..4.0g

NaHSeO3*5H2O .............................................. ..4.0g

pH 7.1 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from Oxoid Unipath.

Caution: Sodium selenite is toxic and a potential
teratogen and care must be taken to avoid inhalation
of the powdered dye, contact with the skin, or inges-
tion, especially in pregnant laboratory workers.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat. Do not autoclave. Dis-
tribute into sterile tubes in 10.0mL volumes. Sterilize for 10 min at 0 psi pressure-100oC.

Use: For the isolation and cultivation of Salmonella typhi and Salmonella paratyphi.


Selenite Cystine Broth

Composition per liter:

Na2HPO4........................................................ ..10.0g

Pancreatic digest of casein................................. .5.0g

Lactose............................................................... .4.0g

Na2SeO3*5H2O.................................................. .4.0g

L-Cystine ........................................................ ..0.02g

pH 7.0 ± 0.2 at 25oC

Source: This medium is available as a premixed
powder from  BD Diagnostic Systems and Oxoid Un-
ipath.

Caution: Sodium selenite is toxic and a potential
teratogen and care must be taken to avoid inhalation
of the powdered dye, contact with the skin, or inges-
tion, especially in pregnant laboratory workers.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat. Do not autoclave. Dis- 

tribute into sterile tubes in 10.0mL volumes. Sterilize

for 15 min at 0 psi pressure-100oC.

Use: For the isolation and cultivation of Salmonella species from feces and other specimens.


Selenite F Broth

Composition per liter:

KH2PO4........................................................... ...7.0g

Pancreatic digest of casein.................................. .5.0g

Lactose............................................................... .4.0g

Na2SeO3*5H2O.................................................. .4.0g

Na2HPO4........................................................... .3.0g

pH 7.0 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Caution: Sodium selenite is toxic and a potential
teratogen and care must be taken to avoid inhalation
of the powdered dye, contact with the skin, or inges-
tion, especially in pregnant laboratory workers.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat. Do not autoclave. Dis-
tribute into sterile tubes in 10.0mL volumes. Sterilize for 30 min at 0 psi pressure-100oC.

Use: For the isolation and cultivation of Salmonella species from feces and other specimens.


Semisolid Brucella Broth

Composition per liter:

Peptone ........................................................... .10.0g

Pancreatic digest of casein............................... ..10.0g

NaCl.................................................................. ..5.0g

Yeast extract...................................................... ..2.0g

Agar ................................................................. ..1.6g

D-Glucose........................................................ ...1.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure-121oC.

Use: For the cultivation of Campylobacter species.


Semisolid Medium for Motility

Composition per liter:

Biosate .............................................................. .5.0g

Polypeptone ..................................................... ..5.0g

NaCl.................................................................. ..5.0g

Agar ................................................................. ..4.0g

Myosate........................................................... ...1.5g

Triphenyltetrazolium chloride solution .......... . 2.5mL

pH 6.9-7.1at 25oC

Triphenyltetrazolium Chloride Solution:

Composition per 10.0mL:

Triphenyltetrazolium chloride ........................... .0.1g

Ethanol (95% solution).............................. ...10.0mL

Preparation of Triphenyltetrazolium Chlo-
ride Solution: Add triphenyltetrazolium chloride to 10.0mL of ethanol. Mix thoroughly.

Preparation of Medium: Add components, ex-
cept triphenyltetrazolium chloride solution, to dis-
tilled/deionized water and bring volume to 997.5mL.
Mix thoroughly. Gently heat and bring to boiling.
Add 2.5mL of triphenyltetrazolium chloride solu-
tion. Mix thoroughly. Distribute into tubes in 10.0mL
volumes. Autoclave for 15 min at 15 psi pressure-
121oC.

Use: For the differentiation of bacteria based on mo-
tility.


Sensitest Agar

Composition per liter:

Pancreatic digest of casein.............................. ..11.0g

Agar ................................................................. ..8.0g

Buffer salts........................................................ .3.3g

Peptone.............................................................. .3.0g

NaCl................................................................. ..3.0g

Glucose ........................................................... ...2.0g

Starch .............................................................. ...1.0g

Nucleoside bases............................................ ..0.02g

Thiamine...................................................... ..0.02mg

pH 7.4 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure-121oC. Pour into sterile Petri dish-
es.

Use: For the performance of antibiotic sensitivity as-
says.


Serratia Differential Medium

(SD Medium)

Composition per 102.0mL:

Solution A.................................................. ..92.0mL

Solution B .................................................. ..10.0mL

pH 6.7 ± 0.2 at 25oC

Solution A:

Composition per 92.0mL:

Yeast extract..................................................... ..1.0g

L-Ornithine ........................................................ .1.0g

NaCl................................................................. ..0.5g


 

Agar ................................................................. ..0.4g

Irgasan inhibitor............................................. . 1.0mL

Indicator solution.......................................... .. 1.0mL

Preparation of Solution A: Add components to
distilled/deionized  water  and  bring  volume  to
92.0mL. Mix thoroughly. Adjust pH to 6.7 with 1N
NaOH.

Irgasan Inhibitor:

Composition per 100.0mL:

Irgasan-DP-300 (4,2ด, 4ด-trichloro-

2-hydroxydiphenylether)........................... ...0.1g

NaOH (1N solution)................................... .. 10.0mL

Preparation of Irgasan Inhibitor: Add irgasan to 10.0mL of NaOH solution. Mix thoroughly. Gen-
tly heat to dissolve. Bring volume to 100.0mL with distilled/deionized water.

Indicator Solution:

Composition per 100.0mL:

Bromthymol Blue ............................................. ..0.2g

Phenol Red......................................................... .0.1g

Preparation of Indicator Solution: Add com-
ponents to 50.0mL of distilled/deionized water. Mix thoroughly for 1h. Bring volume to 100.0mL with distilled/deionized water.

Solution B:

Composition per 10.0mL:

L-Arabinose...................................................... ...1.0g

Preparation of Solution B: Add L-arabinose to distilled/deionized  water  and  bring  volume  to 10.0mL. Mix thoroughly.

Preparation of Medium: Combine 92.0mL of
solution A with 10.0mL of solution B. Mix thorough-
ly. Distribute into tubes. Autoclave for 15 min at 15
psi pressure-121oC. Allow tubes to cool in an up-
right position.

Use: For the cultivation and differentiation of Serratia
species based on the fermentation of arabinose and pro-
duction of ornithine decarboxylase. Serratia marce-
scens changes the medium to purple throughout the
tube. Serratia liquefaciens changes the medium to a
band of purple at the top of the tube with a green/yellow
butt. Serratia rubidaea changes the medium to yellow
throughout the tube.


Serratia Hd-MHr

Composition per liter:

Agar .............................................................. ...15.0g

K2HPO4........................................................... ...7.0g

Glucose ........................................................... ...5.0g

KH2PO4........................................................... ...3.0g

2-Methyl-DL-histidine*2HCl ............................. .1.0g

(NH4)2SO4........................................................ ..1.0g

MgSO4*7H2O.................................................. ...0.5g

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure-121oC. Pour into sterile Petri dish-
es or leave in tubes.

Use: For the cultivation and maintenance of Serratia marcescens.


Serratia Medium

(ATCC Medium 181)

Composition per liter:

Agar .............................................................. ...20.0g

Pancreatic digest of casein................................. .5.0g

Yeast extract..................................................... ..5.0g

Glucose ........................................................... ...1.0g

K2HPO4........................................................... ...1.0g

pH 7.0 ± 0.2 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure-121oC. Pour into sterile Petri dish-
es or leave in tubes.

Use: For the cultivation and maintenance of Serratia marcescens.


Serratia Medium

(ATCC Medium 1399)

Composition per liter:

Agar .............................................................. ...15.0g

K2HPO4........................................................... ...7.0g

Glucose ........................................................... ...5.0g

KH2PO4........................................................... ...3.0g

Casein hydrolysate............................................. .1.0g

(NH4)2SO4........................................................ ..1.0g

Yeast extract..................................................... ..1.0g

MgSO4*7H2O.................................................. ...0.1g

pH 7.0 ± 0.2 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure-121oC. Pour into sterile Petri dish-
es or leave in tubes.

Use: For the cultivation and maintenance of Serratia marcescens.


 

Serum Glucose Agar

(Serum Dextrose Agar)

(ATCC Medium 287)

Composition per 1060.0mL:

Agar .............................................................. ...15.0g

Peptone ........................................................... .10.0g

Beef extract...................................................... ...5.0g

NaCl.................................................................. ..5.0g

Serum-glucose solution .............................. .. 60.0mL

pH 7.3 ± 0.2 at 25oC

Serum-Glucose Solution:

Composition per 60.0mL:

D-Glucose......................................................... .10.0g

Serum (inactivated at 56oC, 30 min) ......... ... 50.0mL

Preparation of Serum-Glucose Solution: Add
glucose to 50.0mL of heat-inactivated serum. Horse
serum or ox serum may be used. Mix thoroughly. Fil-
ter sterilize.

Preparation of Medium: Add components, ex-
cept serum-glucose solution, to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 10 psi pressure-115oC. Cool to 50oC. Asepti-
cally add 60.0mL of sterile serum-glucose solution.
Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes. Allow tubes to cool in a
slanted position.

Use: For the cultivation and maintenance of Brucel-
la species.


Serum Glucose Agar, Farrell Modified

Composition per 1086.9mL:

Agar .............................................................. ...15.0g

Peptone ........................................................... .10.0g

Be