SABHI Agar

(Sabouraud Glucose and

Brain Heart Infusion Agar)

Composition per liter:

Glucose ........................................................... .21.0g

Agar .............................................................. ...15.0g

Pancreatic digest of casein............................... ..10.5g

Peptic digest of animal tissue ........................... ..5.0g

Brain heart, solids from infusion ..................... ...4.0g

NaCl.................................................................. ..2.5g

Na2HPO4........................................................ ..1.25g

pH 6.8 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC. Pour into sterile Petri dish-
es in 20.0mL volumes or leave in tubes.

Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens.


SABHI Agar

Composition per liter:

Beef heart, infusion from.............................. ..125.0g

Calf brains, infusion from.............................. .100.0g

Glucose ........................................................... .21.0g

Agar .............................................................. ...15.0g

Neopeptone...................................................... ...5.0g

Proteose peptone................................................ .5.0g

NaCl.................................................................. ..2.5g

Na2HPO4........................................................ ..1.25g

Chloromycetin solution ................................. . 1.0mL

pH 7.0 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Chloromycetin Solution:

Composition per 10.0mL:

Chloromycetin ................................................... .1.0g

Preparation of Chloromycetin Solution: Add
chloromycetin to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept chloromycetin solution, to distilled/deionized water and bring volume to 999.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121oC. Cool to 45o-50oC. Aseptically add 1.0mL of sterile chloromycetin solu-
tion. Mix thoroughly. Aseptically distribute into ster-
ile tubes in 5.0mL volumes.

Use: For the cultivation of dermatophytes and other

pathogenic and nonpathogenic fungi from clinical and nonclinical specimens.


SABHI Agar, Modified

Composition per liter:

Beef heart, infusion from................................. .62.5g

Calf brain, infusion from ................................ .50.0g

Glucose ........................................................... .20.5g

Brain heart infusion broth.............................. ...18.6g

Agar ................................................................. ..7.5g

Neopeptone...................................................... ...5.0g

Pancreatic digest of gelatin............................... ...2.5g

NaCl.............................................................. ...1.25g

Na2HPO4..................................................... ...0.625g

pH 6.8 ± 0.2 at 25oC

Preparation of Medium: Dissolve, then auto-
clave at 15 psi pressure-121oC for 15 min. Cool to
50oC and add 1.0mL of sterile chloramphenicol solu-
tion (100.0mg/mL). Mix well and dispense into sterile
tubes. Slant and allow to harden. Refrigerate until
needed.

Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens.


SABHI Blood Agar

Composition per liter:

Beef heart, infusion from.............................. ..125.0g

Calf brains, infusion from.............................. .100.0g

Glucose ........................................................... .21.0g

Agar .............................................................. ...15.0g

Neopeptone...................................................... ...5.0g

Proteose peptone................................................ .5.0g

NaCl................................................................. ..2.5g

Na2HPO4........................................................ ..1.25g

Blood........................................................ ..100.0mL

Chloromycetin solution................................. ..1.0mL

pH 7.0 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Chloromycetin Solution:

Composition per 10.0mL:

Chloromycetin .................................................. .1.0g

Preparation of Chloromycetin Solution: Add
chloromycetin to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except
blood and chloromycetin solution, to distilled/deionized
water and bring volume to 899.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure-121oC. Cool to 45o-50oC. Aseptical-
ly add 100.0mL of sterile blood and 1.0mL of sterile

chloromycetin solution. Sheep blood or human blood

may be used. Mix thoroughly. Aseptically distribute into sterile tubes in 5.0mL volumes.

Use: For the cultivation of dermatophytes and other
pathogenic and nonpathogenic fungi from clinical
and nonclinical specimens. Blood enhances the re-
covery of Blastomyces dermatitidis and Histoplasma
capsulatum and their conversion to the yeast phase.


Sabouraud Agar

Composition per liter:

Neopeptone...................................................... .30.0g

Agar .............................................................. ...20.0g

Preparation of Medium: Add components to tap
water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure-
121oC. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of yeasts and molds.


 

Sabouraud Agar with

CCG and 3% NaCl

Composition per 3031.5mL:

Glucose ........................................................ ..120.0g

NaCl............................................................... ...90.0g

Agar .............................................................. ...45.0g

Peptone ........................................................... .30.0g

Chloramphenicol solution........................... .. 15.0mL

Cycloheximide solution............................... .. 15.0mL

Gentamicin solution....................................... . 1.5mL

Chloramphenicol Solution:

Composition per 15.0mL:

Chloramphenicol............................................. ..0.15g

Preparation of Chloramphenicol Solution:

Add chloramphenicol to distilled/deionized water
and bring volume to 15.0mL. Mix thoroughly. Filter
sterilize.

Cycloheximide Solution:

Composition per 15.0mL:

Cycloheximide................................................... .0.3g

Preparation of Cycloheximide Solution: Add
cycloheximide to distilled/deionized water and bring
volume to 15.0mL. Mix thoroughly. Filter sterilize.

Gentamicin Solution:

Composition per 10.0mL:

Gentamicin......................................................... .0.4g

Preparation of Gentamicin Solution: Add
gentamicin to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex-

cept chloramphenicol solution, cycloheximide solu-
tion, and gentamicin solution—to distilled/deionized water and bring volume to 3.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121oC. Cool to 45o-50oC. Aseptically add 15.0mL of sterile chloramphenicol solution, 15.0mL of sterile cycloheximide solution, and 1.5mL of sterile gentamicin solution. Mix thor-
oughly. Aseptically distribute into sterile tubes. Al-
low tubes to cool in a slanted position.

Use: For the selective isolation and cultivation of fungi from specimens with a mixed flora.


Sabouraud Agar with

CCG and 5% NaCl

Composition per 3031.5mL:

NaCl.............................................................. .150.0g

Glucose ........................................................ ..120.0g

Agar .............................................................. ...45.0g

Peptone........................................................... ..30.0g

Chloramphenicol solution........................... ..15.0mL

Cycloheximide solution.............................. ..15.0mL

Gentamicin solution....................................... .1.5mL

Chloramphenicol Solution:

Composition per 15.0mL:

Chloramphenicol............................................. ..0.15g

Preparation of Chloramphenicol Solution:

Add chloramphenicol to distilled/deionized water
and bring volume to 15.0mL. Mix thoroughly. Filter
sterilize.

Cycloheximide Solution:

Composition per 15.0mL:

Cycloheximide................................................... .0.3g

Preparation of Cycloheximide Solution: Add
cycloheximide to distilled/deionized water and bring
volume to 15.0mL. Mix thoroughly. Filter sterilize.

Gentamicin Solution:

Composition per 10.0mL:

Gentamicin......................................................... .0.4g

Preparation of Gentamicin Solution: Add
gentamicin to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components—ex-
cept chloramphenicol solution, cycloheximide solu-
tion, and gentamicin solution—to distilled/deionized
water and bring volume to 3.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure-121oC. Cool to 45o-50oC.
Aseptically add 15.0mL of sterile chloramphenicol
solution, 15.0mL of sterile cycloheximide solution,
and 1.5mL of sterile gentamicin solution. Mix thor-


 

oughly. Aseptically distribute into sterile tubes. Al-
low tubes to cool in a slanted position.

Use: For the selective isolation and cultivation of fungi from specimens with a mixed flora.


Sabouraud Glucose Agar, Emmons

Composition per liter:

Glucose ........................................................... .20.0g

Agar .............................................................. ...17.0g

Pancreatic digest of casein.................................. .5.0g

Peptic digest of animal tissue ........................... ..5.0g

pH 6.9 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 13 psi pressure-118oC. Pour into sterile Petri dish-
es or leave in tubes.

Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. For the cultivation of yeast and filamentous fungi.


Sabouraud Glucose Broth

Composition per liter:

Glucose ........................................................... .20.0g

Neopeptone...................................................... .10.0g

pH 5.6 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure-121oC. Avoid
overheating.

Use: For the cultivation of pathogenic and nonpatho-
genic fungi, especially dermatophytes. The medium may be made more selective for fungi by the addition of chloramphenicol.


Sabouraud Maltose Agar

Composition per liter:

Maltose ........................................................... .40.0g

Agar .............................................................. ...15.0g

Pancreatic digest of casein.................................. .5.0g

Peptic digest of animal tissue ........................... ..5.0g

pH 5.6 ± 0.2 at 25oC

Source: This medium is available as a premixed

powder from  BD Diagnostic Systems and Oxoid Un-
ipath.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC. Avoid overheating. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of a vari-
ety of fungi.


Sabouraud Maltose Broth

Composition per liter:

Maltose........................................................... ..40.0g

Neopeptone...................................................... .10.0g

pH 5.6 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure-121oC. Avoid
overheating.

Use: For the cultivation of a variety of fungi.