รวมอาหารเลี้ยงเชื้อหมวด R

posted on 04 Jun 2012 22:32 by moomsabuy in medium directory Knowledge

 


R Medium

 

Composition per liter:

Agar .............................................................. ...30.0g

NaHCO3........................................................... ..8.0g

K2HPO4........................................................... ...3.0g

Glucose ........................................................... ...2.5g

Glutamine........................................................ .0.61g

Serine .............................................................. .0.24g

Leucine........................................................... ..0.23g

Lysine.............................................................. .0.23g

Asparagine ..................................................... ..0.18g

Valine ............................................................. .0.17g

Isoleucine......................................................... .0.17g

Tyrosine ........................................................ ...0.14g

Arginine*HCl............................................... ...0.125g

Phenylalanine................................................ ..0.125g

Threonine......................................................... .0.12g

Methionine................................................... ...0.073g

Glycine........................................................ ...0.065g

Histidine*HCl............................................... ..0.055g

Proline........................................................... .0.043g

Tryptophan.................................................. ...0.035g

L-Cystine..................................................... ...0.025g

MgSO4*H2O.................................................. ..9.9mg

CaCl2*2H2O.................................................. ...7.4mg

Adenine sulfate ............................................ ...2.1mg

Uracil ........................................................... ...1.4mg

Thiamine*HCl ............................................... ..1.0mg

MnSO4*H2O.................................................. ..0.9mg

pH 8.0 ± 0.2 at 25oC

Preparation of Medium: Add components, ex-
cept agar, to distilled/deionized water and bring vol-
ume to 500.0mL. Mix thoroughly. Filter sterilize.
Warm to 45o-50oC. Add agar to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure-121oC. Cool
to 45o-50oC. Aseptically combine both solutions.
Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.

Use: For the cultivation of Bacillus anthracis.


Rabbit Blood Agar

Composition per 1250.0mL:

Pancreatic digest of casein............................... ..16.0g

Agar .............................................................. ...13.5g

Brain heart, solids from infusion ..................... ...8.0g

Peptic digest of animal tissue ........................... ..5.0g

NaCl.................................................................. ..5.0g

Na2HPO4........................................................... .2.5g

Glucose ........................................................... ...2.0g

Rabbit blood, defibrinated ........................ .. 250.0mL

pH 7.4 ± 0.2 at 25oC

Preparation of Medium: Add components, ex-
cept rabbit blood, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Autoclave for
15 min at 15 psi-121oC. Aseptically add sterile rab-
bit blood. Pour into sterile Petri dishes or aseptically
distribute into sterile tubes or flasks while shaking.

Use: For the cultivation and maintenance of Coryne-
bacterium diphtheriae, Haemophilus ducreyi, and Actinobacillus lignieresii.


Rabbit Heart Infusion Agar

Composition per liter:

Beef heart, infusion from.............................. ..500.0g

Agar .............................................................. ...15.0g

Tryptose ........................................................ ...10.0g

NaCl.................................................................. ..5.0g

Rabbit blood, defibrinated ........................... . 50.0mL

pH 7.4 ± 0.2 at 25oC

Preparation of Medium: Add components, except rabbit blood, to distilled/deionized water and bring vol-
ume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure-121oC.  Cool  to 50o-55oC.  Aseptically  add

50.0mL of sterile rabbit blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the culture of Bartonella quintana.

Rabbit Serum Medium

(Rabbit Serum Bovine Serum

Albumin Tween 80 Medium)

(Rabbit Serum BSA

Tween 80 Medium)

Composition per liter:

Basal medium .......................................... ... 900.0mL

Rabbit serum with supplements............... ... 100.0mL

pH 7.4 ± 0.2 at 25oC

Basal Medium:

Composition per 900.0mL:

Na2HPO4........................................................... .1.0g

NaCl.................................................................. ..1.0g

KH2PO4........................................................... ...0.3g

Glycerol (10% solution)................................ .1.0mL

NH4Cl (25% solution)................................... .1.0mL

Sodium pyruvate (10% solution).................. ..1.0mL

Thiamine (0.5% solution) ............................. .1.0mL

Preparation of Basal Medium: Add compo-
nents to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 20 min at 15 psi pressure-
121oC. Cool to 25oC.

Rabbit Serum with Supplements:

Composition per 106.0mL:

Rabbit serum............................................. ..100.0mL

L-Asparagine (3% solution) .......................... .5.0mL

MgCl2-CaCl2 solution.................................... .1.0mL

Preparation of Rabbit Serum with Supple-
ments: Combine the three solutions. Mix thorough-
ly. Filter sterilize.

MgCl2-CaCl2 Solution:

Composition per 100.0mL:

CaCl2*2H2O..................................................... ...1.5g

MgCl2*6H2O .................................................... .1.5g

Preparation of MgCl2-CaCl2 Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Aseptically combine
900.0mL of cooled sterile basal medium and 100.0mL
of sterile rabbit serum with supplements. Mix thorough-
ly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Leptospira species.

Rainbow Agar O157

Composition per liter:

Proprietary.

Source: This medium is available as a premixed powder from Biolog Inc.

Preparation: Suspend 60.0g of the proprietary
mixture in distilled/deionized water and bring vol-
ume to 1.0L Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure-
121oC. Cool to 45-50oC. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes. The
final medium should be clear and virtually colorless.
No pH adjustment is needed. The final pH should be
pH 7.9-8.3. To increase the selectivity of the medi-
um, a sterile solution containing 0.8 mg potassium
tellurite and 10 mg novobiocin can be added. Caution
must be used because tellurite is toxic.

Use: For the detection, isolation, and presumptive
identification of verotoxin-producing strains of Es-
cherichia coli, particularly serotype O157:H7. The
medium contains chromogenic substrates that are 

specific for two E. coli-associated enzymes: β-galac-

tosidase (a blue-black chromogenic substrate) and β-
glucuronidase (a red chromogenic substrate). The
distinctive  black  or  gray  coloration  of  E.  coli
O157:H7 colonies is easily viewed by laying the Petri
plate against a white background. When O157 is sur-
rounded by pink or magenta non-toxigenic colonies,
it may have a bluish hue. The addition of selective
agents improves performance. E. coli O157:H7 colo-
ny coloration will be slightly bluer with these selec-
tive agents added.Tellurite is highly selective for E.
coli O157:H7 and can reduce background flora con-
siderably. Novobiocin inhibits Proteus swarming and
the growth of tellurite-reducing bacteria. Rare strains
of O157:H7 are tellurite sensitive5.


RAMBACH Agar

Composition per liter:

NaC.................................................................. ..l5.0g

Agar .............................................................. ...15.0g

Proplylene glycol............................................. .10.5g

Peptone ........................................................... ...8.0g

Chromogenic mix ............................................. ..1.5g

Sodium deoxycholate....................................... ...1.0g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available from Merck.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Heat in a boiling water-batch or in a
current of steam, while shaking from time to time.
The medium is totally suspended, if no visual parti-
cles stick to the glass-wall. The medium should not
be heat-treated further. Complete dissolution with
shaking in 5-min. sequences is approximately 35-40
min. Do not autoclave. Do not overheat. Cool as fast
as possible to 45o-50oC while gently shaking from
time to time. Pour into sterile Petri dishes. To prevent
any precipitate or clotting of the chromogenic-mix in
the plates, place Petri dishes during pouring proce-
dure on a cool (max. 25oC) surface. The plates are
opaque and pink.

Use: For the detection of enteric bacteria, including
coliforms and Salmonella spp. Sodium desoxycho-
late inhibits the accompanying Gram-positive flora.
This medium enables Salmonella spp. to be differen-
tiated unambiguously from other bacteria. Salmonel-
la spp. form a characteristic red color. In order to dif-
ferentiate coliforms from Salmonellae, the medium
contains a chromogene indicating the presence of β-
galactosidase splitting, a characteristic for coliforms.
Coliform microorganisms grow as blue-green or
blue-violet colonies. Other Enterobacteriaceae and
Gram-negative bacteria, such as Proteus, Pseudomo-

nas, Shigella, S. typhi and S. parathyphi A grow as

colorless-yellow colonies.

Rapid Fermentation Medium

Composition per liter:

Pancreatic digest of casein.............................. ..20.0g

NaCl................................................................. ..5.0g

Agar ................................................................. ..3.5g

L-Cystine........................................................... .0.5g

Na2SO3.............................................................. .0.5g

Phenol Red.................................................. ...0.017g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure-121oC.

Use: For the differentiation of Neisseria species iso-
lated from clinical specimens.


RAPIDดE. coli 2 Agar

Composition per liter:

Proprietary

Source: This medium is available from Biorad.

Use: For the direct enumeration of E.coli and
coliforms. Selectivity and electivity are based on the
detection of glucuronidase and galactosidase activi-
ties. Hydrolysis of chromogenic substrate results in
purple to pink E.coli colonies (gluc+/gal+) and blue-
green coliform colonies (gluc-/gal+). RAPIDดE.coli
2 agar is AFNOR validated according to ISO 16140
protocol to enumerate E.coli and coliforms on the
same plate at 37oC, without any further confirmation
of characteristic colonies.

RAPIDดEnterococcus Agar

Composition per liter:

Proprietary

Source: This medium is available from Biorad.
Use: For the direct enumeration, without confirma-
tion, of enterococci. The cleavage of the chromogen-
ic substrate by glucosidase activity of Enterococci
leads to specific blue colonies. RAPIDดEnterococcus
totally inhibits growth of Gram-negative flora and
that of practically all Gram-positive bacteria other
than Enterococci, due to the combined action of tem-
perature and selective media.

RAPIDดL. mono Medium

Composition per liter:

Peptones........................................................ ...30.0g

Agar B, proprietary.......................................... .13.0g

D-Xylose ........................................................ ..10.0g

LiCl.................................................................. ...9.0g

Meat extract ...................................................... ..5.0g

Yeast extract...................................................... ..1.0g

Phenol Red...................................................... ..0.12g

Selective supplement, proprietary ................... ..20.0g

Chromogenic substrate, proprietary............... . 1.0mL

pH 7.3 ± 0.1 at 25oC

Source: This medium is available from Biorad.
Use: For the detection and differentiation of Listeria
spp., including L. ivanovii and L. monocytogenes.


Rappaport Broth, Modified

(Rap Broth, Modified)

Composition per 250.2mL:

Solution A................................................ ... 155.0mL

Solution C................................................... .. 53.0mL

Solution B................................................... .. 40.0mL

Solution D...................................................... . 1.6mL

Solution E ..................................................... . 0.6mL

Solution A:

Composition per liter:

Pancreatic digest of casein............................... ..10.0g

Preparation of Solution A: Add pancreatic di-
gest of casein to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Solution B:

Composition per liter:

Na2HPO4........................................................... .9.5g

Preparation of Solution B: Add Na2HPO4 to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Solution C:

Composition per 100.0mL:

MgCl2*6H2O .................................................. ..40.0g

Preparation of Solution C: Add MgCl2*6H2O to distilled/deionized  water  and  bring  volume  to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121oC. Cool to 25oC.

Solution D:

Composition per 100.0mL:

Malachite Green................................................ ..0.2g

Preparation  of  Solution  D:  Add  Malachite
Green to sterile distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Do not steril-
ize.

Solution E:

Composition per 10.0mL:

Carbenicillin ................................................... ..0.01g

Preparation of Solution E: Add carbenicillin to

distilled/deionized  water  and  bring  volume  to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Combine 155.0mL of solution A and 40.0mL of solution B. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure-121oC. Cool to 45o-50oC. Aseptically add 53.0mL of sterile solution C, 1.6mL of solution D, and 0.6mL of sterile solution E. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Yersinia en-
terocolitica.

Rappaport-Vassiliadis

Enrichment Broth

(RV Enrichment Broth)

Composition per 1110.0mL:

NaCl................................................................. ..8.0g

Papaic digest of soybean meal........................... .5.0g

KH2PO4........................................................... ...1.6g

Magnesium chloride solution..................... .100.0mL

Malachite Green solution........................... ...10.0mL

pH 5.2 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from Oxoid Unipath.

Magnesium Chloride Solution:

Composition per 100.0mL:

MgCl2*6H2O ................................................. ..40.0g

Preparation of Magnesium Chloride Solu-
tion: Add MgCl2*6H2O to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure-121oC. Cool to
45o-50oC.

Malachite Green Solution:

Composition per 10.0mL:

Malachite Green oxalate.................................. ..0.04g

Preparation of Malachite Green Solution:

Add Malachite Green to distilled/deionized water
and bring volume to 10.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure-121oC. Cool to
45o-50oC.

Preparation of Medium: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 10 psi pressure-115oC.

Use: For the isolation and cultivation of Salmonella species.

Rappaport-Vassiliadis R10 Broth Composition per liter:

MgCl2, anhydrous......................................... ...13.4g

NaCl................................................................. ..7.2g 

Papaic digest of soybean meal......................... ..4.54g

KH2PO4........................................................... .1.45g

Malachite Green oxalate............................... ...0.036g

pH 5.1 ± 0.2 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into screw-capped tubes
in 10.0mL volumes. Autoclave for 15 min at 10 psi
pressure-116oC.

Use: For the isolation and cultivation of Salmonella species.


Rappaport-Vassiliadis

Soya Peptone Broth

(RVS Broth)

Composition per liter:

MgCl2, anhydrous.......................................... .13.58g

NaCl.................................................................. ..7.2g

Papaic digest of soybean meal............................ .4.5g

KH2PO4........................................................... .1.26g

K2HPO4........................................................... .0.18g

Malachite Green............................................. .0.036g

pH 5.2 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into screw-capped tubes
in 10.0mL volumes. Autoclave for 15 min at 10 psi
pressure-115oC.

Use: For the isolation and cultivation of Salmonella species.


Reactivation with Tryptone Soya Broth

(DSMZ Medium 220a)

Composition per liter:

Peptone from casein....................................... ...17.0g

Peptone from soymeal ....................................... .3.0g

NaCl.................................................................. ..5.0g

D(+)-Glucose..................................................... .2.5g

K2HPO4........................................................... ...2.5g

pH 7.3 ± 0.2 at 25oC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC.

Use: For the reactivation and cultivation of Aeromo-
nas spp. and Burkholderia spp.


Regan-Lowe Semisolid

Transport Medium

Composition per liter:

Agar ................................................................. ..6.0g

Beef extract...................................................... ...5.0g

Pancreatic digest of gelatin............................... ...5.0g

Soluble starch................................................... ...5.0g

NaCl.................................................................. ..2.5g

Charcoal............................................................ ..2.0g

Niacin.............................................................. .0.01g

Horse blood, defibrinated ....................... ...100.0mL

Cephalexin solution ................................... ..10.0mL

pH 7.4 ± 0.2 at 25oC

Cephalexin Solution:

Composition per 10.0mL:

Cephalexin ..................................................... ..0.04g

Preparation  of  Cephalexin  Solution:  Add cephalexin to distilled/deionized water and bring vol-
ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept horse blood and cephalexin solution, to distilled/
deionized water and bring volume to 890.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure-121oC. Cool to
45o-50oC. Aseptically add sterile horse blood and
sterile cephalexin solution. Mix thoroughly. Asepti-
cally distribute into small, sterile, screw-capped
tubes. Fill tubes half-full. Swirl medium while dis-
pensing to keep charcoal in suspension.

Use: For the transport of Bordetella pertussis and
Bordetella parapertussis isolated from clinical spec-
imens.


Reinforced Clostridial Agar

Composition per liter:

Agar .............................................................. ...13.5g

Beef extract...................................................... .10.0g

Pancreatic digest of casein.............................. ..10.0g

NaCl................................................................. ..5.0g

Glucose ........................................................... ...5.0g

Yeast extract..................................................... ..3.0g

Sodium acetate................................................... .3.0g

Soluble starch.................................................. ...1.0g

L-Cysteine*HCl*H2O........................................ ..0.5g

pH 6.8 ± 0.2 at 25oC

Source: This medium is available as a premixed
powder from  BD Diagnostic Systems and Oxoid Un-
ipath.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 10 psi pressure-115oC. Pour into sterile Petri dish-
es or leave in tubes.

Use:  For  the  cultivation  and  enumeration  of Clostridium species, Bifidobacterium species, other anaerobes (e.g., lactobacilli), and facultative organ-
isms from clinical specimens.


Reinforced Clostridial Medium

 Composition per liter:

Tryptose ........................................................ ...10.0g

Beef extract...................................................... .10.0g

Glucose ........................................................... ...5.0g

NaCl.................................................................. ..5.0g

Yeast extract...................................................... ..3.0g

Sodium acetate................................................... .3.0g

Soluble starch................................................... ...1.0g

L-Cysteine*HCl*H2O......................................... .0.5g

Agar ................................................................. ..0.5g

pH 6.8 ± 0.2 at 25oC

Source: This medium is available as a premixed
powder from  BD Diagnostic Systems and Oxoid Un-
ipath.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 10 psi pressure-115oC. Pour into sterile Petri dish-
es or leave in tubes.

Use: For the nonselective cultivation and enumera-
tion of Clostridium species, other anaerobes such as
lactobacilli, and facultative organisms from clinical
specimens.


Reinforced Clostridial Medium

with Sodium Lactate

Composition per liter:

Tryptose ........................................................ ...10.0g

Beef extract...................................................... .10.0g

Glucose ........................................................... ...5.0g

NaCl.................................................................. ..5.0g

Yeast extract...................................................... ..3.0g

Sodium acetate................................................... .3.0g

Soluble starch................................................... ...1.0g

L-Cysteine*HCl*H2O......................................... .0.5g

Agar ................................................................. ..0.5g

Sodium lactate (60% solution)..................... . 15.0mL

pH 6.8 ± 0.2 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 10 psi pressure-115oC. Pour into sterile Petri dish-
es or leave in tubes.

Use: For the nonselective cultivation and enumera-
tion of Clostridium species, other anaerobes such as lactobacilli, from clinical specimens.


Rice Extract Agar

Composition per liter:

Agar .............................................................. ...20.0g

White rice, solids from extract............................ .5.0g

Polysorbate 80 ............................................ . 10.0mL

pH 6.6 ± 0.2 at 25oC

Source: This medium is available as a premixed

powder from  BD Diagnostic Systems.

Preparation of Medium: Add components, ex-
cept polysorbate 80, to distilled/deionized water and
bring volume to 990.0mL. Mix thoroughly. Gently
heat and bring to boiling. Add polysorbate 80. Mix
thoroughly. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure-121oC. Pour into sterile
Petri dishes.

Use: For the cultivation and differentiation of Can-
dida albicans and Candida stellatoidea from other
Candida species based on chlamydospore formation.


Rice Extract Agar

Composition per liter:

Agar .............................................................. ...20.0g

White rice, solids from extract........................ ..20.0g

pH 7.1 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components, ex-
cept polysorbate 80, to distilled/deionized water and
bring volume to 990.0mL. Mix thoroughly. Gently
heat and bring to boiling. Add polysorbate 80. Mix
thoroughly. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure-121oC. Pour into sterile
Petri dishes.

Use: For the cultivation and differentiation of Can-
dida albicans and Candida stellatoidea from other
Candida species based on chlamydospore formation.


Rimler-Shotts Medium

(RS Medium)

Composition per liter:

Agar .............................................................. ...13.5g

Na2S2O3*5H2O ................................................. .6.8g

L-Ornithine*HCl ............................................... ..6.5g

NaCl................................................................. ..5.0g

L-Lysine*HCl..................................................... .5.0g

Maltose.............................................................. .3.5g

Yeast extract..................................................... ..3.0g

Sodium deoxycholate....................................... ...1.0g

Ferric ammonium citrate.................................... .0.8g

L-Cysteine*HCl.................................................. .0.3g

Bromthymol Blue ......................................... ...0.03g

Novobiocin solution................................... ..10.0mL

pH 7.0 ± 0.2 at 25oC

Novobiocin Solution:

Composition per 10.0mL:

Novobiocin...          .5.0mg

Preparation of Novobiocin Solution: Add no-
vobiocin to distilled/deionized water and bring vol-
ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept novobiocin solution, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Gen-
tly heat and bring to boiling. Autoclave for 15 min at
15 psi pressure-121oC. Cool to 45o-50oC. Aseptical-
ly add sterile components. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation, cultivation, and pre-
sumptive identification of Aeromonas hydrophila
and other Gram-negative bacteria based on their abil-
ity to decarboxylate lysine and ornithine, ferment
maltose, and produce H2S. Maltose-fermenting bac-
teria appear as yellow colonies. Bacteria that produce
lysine or ornithine decarboxylase turn the medium
greenish-yellow to yellow. Bacteria that produce H2S
appear as colonies with black centers.


RIOT Agar

(Rice Infusion Oxgall Tween 80 Agar)

Composition per 1010.0mL:

Agar .............................................................. ...10.0g

Oxgall ........................................................... ...10.0g

Rice extract....................................................... .. 1.0L

Tween 80..................................................... . 10.0mL

pH 7.3 ± 0.2 at 25oC

Rice Extract:

Composition per liter:

Cream of rice cereal........................................ ...10.0g

Preparation of Rice Extract: Add cream of rice cereal to 1.0L of boiling tap water. Mix thoroughly. Filter quickly through cheesecloth. Bring volume of filtrate to 1.0L with tap water.

Preparation of Medium: Combine components. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC. Pour into sterile Petri dish-
es or leave in tubes.

Use: For the cultivation and differentiation of Can-
dida albicans and Candida stellatoidea from other
Candida species based on chlamydospore formation.


Rogosa Agar

Composition per liter:

Sodium acetate................................................ ..25.0g

Agar .............................................................. ...20.0g

Glucose ........................................................... .20.0g

Pancreatic digest of casein............................... ..10.0g

KH2PO4........................................................... ...6.0g

Yeast extract...................................................... ..5.0g

Ammonium citrate............................................. .2.0g

Sorbitan monooleate ......................................... .1.0g

MgSO4*7H2O............................................... ..0.575g

MnSO4*H2O.................................................. ...0.12g

FeSO4*7H2O................................................. ..0.4mg

Acetic acid, glacial....................................... .1.32mL

pH 5.4 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components, ex-
cept acetic acid, to distilled/deionized water and
bring volume to 998.7mL. Mix thoroughly. Gently
heat and bring to boiling. Add glacial acetic acid.
Mix thoroughly. Gently heat while stirring and bring
to 90o-100oC for 2-3 min. Do not autoclave. Pour
into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation, cultivation, and enumeration of lactobacilli, especially from feces, saliva, and vag-
inal specimens.


Rogosa SL Agar

(Rogosa Selective Lactobacillus Agar)

Composition per liter:

Agar .............................................................. ...15.0g

Sodium acetate................................................ ..15.0g

Glucose ........................................................... .10.0g

Pancreatic digest of casein.............................. ..10.0g

K2HPO4........................................................... ...6.0g

Yeast extract..................................................... ..5.0g

Arabinose........................................................ ...5.0g

Sucrose.............................................................. .5.0g

Ammonium citrate............................................. .2.0g

Sorbitan monooleate ......................................... .1.0g

MgSO4*7H2O.................................................. .0.57g

MnSO4*7H2O.................................................. .0.12g

FeSO4*H2O.................................................... ..0.03g

Acetic acid, glacial....................................... .1.32mL

pH 5.4 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components, ex-
cept glacial acetic acid, to distilled/deionized water
and bring volume to 998.7mL. Mix thoroughly. Gen-
tly heat and bring to boiling. Add glacial acetic acid.
Mix thoroughly. Gently heat while stirring and bring
to 90o-100oC for 2-3 min. Do not autoclave. Pour
into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation, cultivation, and enumeration of lactobacilli, especially from feces, saliva, and vag-
inal specimens.


Rogosa SL Broth

(Rogosa Selective Lactobacillus Broth)

 Composition per liter:

Sodium acetate................................................ ..15.0g

Glucose ........................................................... .10.0g

Pancreatic digest of casein............................... ..10.0g

K2HPO4........................................................... ...6.0g

Yeast extract...................................................... ..5.0g

Arabinose......................................................... ...5.0g

Sucrose.............................................................. .5.0g

Ammonium citrate.............................................. .2.0g

Sorbitan monooleate........................................... .1.0g

MgSO4*7H2O.................................................. .0.57g

MnSO4*7H2O.................................................. .0.12g

FeSO4*H2O..................................................... ..0.03g

Acetic acid, glacial........................................ . 1.32mL

pH 5.4 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components, ex-
cept glacial acetic acid, to distilled/deionized water and bring volume to 998.7mL. Mix thoroughly. Gen-
tly heat and bring to boiling. Add glacial acetic acid. Mix thoroughly. Gently heat while stirring and bring to 90o-100oC for 2-3 min. Do not autoclave. Asepti-
cally distribute into sterile tubes.

Use: For the isolation, cultivation, and enumeration of lactobacilli, especially from feces, saliva, and vag-
inal specimens.


RPMI 1640 Medium with L-Glutamine

Composition per liter:

NaCl.................................................................. ..6.0g

NaHCO3........................................................... ..2.0g

D-Glucose......................................................... ...2.0g

Na2HPO4*7H2O............................................... ...1.5g

KCl..................................................................... .0.4g

L-Glutamine ...................................................... ..0.3g

L-Arginine ........................................................ ..0.2g

Ca(NO3)2*4H2O............................................... ...0.1g

MgSO4*7H2O.................................................. ...0.1g

L-Asparagine................................................... ..0.05g

L-Cystine......................................................... ..0.05g

L-Isoleucine, allo free....................................... .0.05g

L-Leucine, methionine free .............................. .0.05g

L-Lysine*HCl.................................................. ..0.04g

i-Inositol......................................................... .0.035g

L-Serine............................................................ .0.03g

L-Aspartic acid................................................ ..0.02g

L-Glutamic acid................................................ .0.02g

L-Hydroxyproline............................................. .0.02g

L-Proline, hydroxy-L-proline free..................... .0.02g

L-Threonine, allo free....................................... .0.02g

L-Tyrosine........................................................ .0.02g

L-Valine .......................................................... .0.02g

L-Histidine, free base................................... ...0.015g

L-Methionine................................................ ...0.015g

L-Phenylalanine............................................. ..0.015g

Glycine........................................................... ..0.01g

L-Tryptophan.................................................. .5.0mg

Phenol Red..................................................... .5.0mg

Choline chloride............................................. ..3.0mg

Glutathione, reduced....................................... .1.0mg

p-Aminobenzoic acid.................................... ...1.0mg

Folic acid........................................................ .1.0mg

Nicotinamide................................................... .1.0mg

Pyridoxine*HCl ........................................... ...1.0mg

Thiamine*HCl ............................................... ..1.0mg

D-Calcium pantothenate............................... ...0.25mg

Biotin ........................................................... ...0.2mg

Riboflavin ..................................................... ..0.2mg

Vitamin B12 .................................................. ... 5.0ตg

pH 7.3 ± 0.2 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Adjust pH to 7.3 with 1N HCl or 1N NaOH. Filter
sterilize. Aseptically distribute into sterile tubes or
flasks.

Use: For the cultivation of mammalian cells in tissue
culture. Culture media for human immunodeficiency
viruses.


Russell Double-Sugar Agar

Composition per liter:

Agar .............................................................. ...15.0g

Proteose peptone no. 3.................................... ..12.0g

Lactose............................................................ ..10.0g

NaCl................................................................. ..5.0g

Beef extract...................................................... ...1.0g

Glucose ........................................................... ...1.0g

Phenol Red.................................................. ...0.025g

pH 7.5 ± 0.2 at 25oC

Preparation of Medium: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute
into tubes. Autoclave for 15 min at 15 psi pressure-
121oC. Allow tubes to cool in a slanted position.

Use: For the identification of Gram-negative enteric
bacilli based on their fermentation of glucose and
lactose. Bacteria that ferment both glucose and lac-
tose produce a yellow slant and yellow butt. Bacteria
that ferment glucose but do not ferment lactose pro-
duce a red slant and a yellow butt. Bacteria that fer-
ment neither glucose nor lactose produce an un-
changed pink-orange color.