รวมอาหารเลี้ยงเชื้อหมวด P

posted on 04 Jun 2012 22:30 by moomsabuy in medium directory Knowledge

Phenol Red Broth

Composition per liter:

Pancreatic digest of casein.............................. ..10.0g

NaCl................................................................. ..5.0g

Phenol Red Lactose Agar     355

 Phenol Red................................................... ...0.018g

Carbohydrate solution................................. .. 20.0mL

pH 7.4 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Carbohydrate Solution:

Composition per 20.0mL:

Carbohydrate............................................. .5.0-10.0g

Preparation of Carbohydrate Solution: Add
carbohydrate to distilled/deionized water and bring
volume to 20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex-
cept carbohydrate solution, to distilled/deionized wa-
ter and bring volume to 980.0mL. Mix thoroughly.
Adjust pH to 7.4 if necessary. Distribute into tubes
containing an inverted Durham tube. Fill each tube
with 9.8mL of medium. Autoclave for 15 min at 13 psi
pressure-118oC. Cool to 45o-50oC. Aseptically add
0.2mL of sterile carbohydrate solution to each tube.

Use: For the determination of fermentation reactions
in the differentiation of microorganisms. Fermenta-
tion is determined by the production of acid—broth
turns yellow—and formation of gas—bubble trapped
in Durham tube.


Phenol Red Glucose Broth

Composition per liter:

Pancreatic digest of casein............................... ..10.0g

Glucose ........................................................... ...5.0g

NaCl.................................................................. ..5.0g

Phenol Red................................................... ...0.018g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if necessary. Dis-
tribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Auto-
clave for 15 min at 13 psi pressure-118oC.

Use: For determination of the ability of a microor-
ganism to ferment glucose. Fermentation is deter-
mined  by  the  production  of  acid—broth  turns
yellow—and formation of gas—bubble trapped in
Durham tube.


Phenol Red Lactose Agar

Composition per liter:

Agar .............................................................. ...15.0g

Lactose............................................................ ..10.0g

Proteose peptone no. 3.................................... ..10.0g 

NaCl................................................................. ..5.0g

Beef extract...................................................... ...1.0g

Phenol Red.................................................. ..25.0mg

pH 7.4 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 13 psi pressure-118oC. Pour into sterile Petri dish-
es or leave in tubes. Allow tubes to cool in a slanted
position.

Use: For determination of the ability of a microorgan-
ism to ferment lactose. Fermentation is determined by the production of acid—medium turns yellow.


Phenol Red Lactose Broth

Composition per liter:

Pancreatic digest of casein.............................. ..10.0g

Lactose............................................................... .5.0g

NaCl................................................................. ..5.0g

Phenol Red.................................................. ...0.018g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if necessary. Dis-
tribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Auto-
clave for 15 min at 13 psi pressure-118oC.

Use: For determination of the ability of a microor-
ganism to ferment lactose. Fermentation is deter-
mined  by  the  production  of  acid—broth  turns
yellow—and formation of gas—bubble trapped in
Durham tube.


Phenol Red Mannitol Agar

Composition per liter:

Agar .............................................................. ...15.0g

Mannitol........................................................ ...10.0g

Proteose peptone no. 3.................................... ..10.0g

NaCl................................................................. ..5.0g

Beef extract...................................................... ...1.0g

Phenol Red.................................................. ..25.0mg

pH 7.4 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distrib-

ute into tubes or flasks. Autoclave for 15 min at 13 psi
pressure-118oC. Pour into sterile Petri dishes or leave
in tubes. Allow tubes to cool in a slanted position.

Use: For determination of the ability of a microor-
ganism to ferment mannitol. Fermentation is deter-
mined by the production of acid—medium turns
yellow.


Phenol Red Mannitol Broth

Composition per liter:

Pancreatic digest of casein............................... ..10.0g

D-Mannitol......................................................... .5.0g

NaCl.................................................................. ..5.0g

Phenol Red................................................... ...0.018g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if necessary. Dis-
tribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Auto-
clave for 15 min at 13 psi pressure-118oC.

Use: For determination of the ability of a microor-
ganism to ferment mannitol. Fermentation is deter-
mined  by  the  production  of  acid—broth  turns
yellow—and formation of gas—bubble trapped in
Durham tube.


Phenol Red Sucrose Broth

Composition per liter:

Pancreatic digest of casein............................... ..10.0g

NaCl.................................................................. ..5.0g

Sucrose.............................................................. .5.0g

Phenol Red................................................... ...0.018g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if necessary. Dis-
tribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Auto-
clave for 15 min at 13 psi pressure-118oC.

Use: For determination of the ability of a microor-
ganism to ferment sucrose. Fermentation is deter-
mined  by  the  production  of  acid—broth  turns
yellow—and formation of gas—bubble trapped in
Durham tube.


Phenol Red Tartrate Agar

Composition per liter:

Agar .............................................................. ...15.0g

Peptone........................................................... ..10.0g

Potassium tartrate............................................. .10.0g

NaCl................................................................. ..5.0g

Phenol Red.................................................. ...0.024g

pH 7.6 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
cold distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Distribute into tubes or flasks. Autoclave for 15
min at 13 psi pressure-118oC. Pour into sterile Petri
dishes or leave in tubes. Allow tubes to cool in an up-
right position.

Use: For the differentiation of Gram-negative bacte-
ria of the intestinal groups, particularly members of the Salmonella (paratyphoid) group based on their ability to ferment tartrate.


Phenol Red Tartrate Broth

Composition per liter:

Pancreatic digest of casein.............................. ..10.0g

Potassium tartrate............................................. .10.0g

Agar ................................................................. ..5.0g

NaCl................................................................. ..5.0g

Phenol Red.................................................. ...0.024g

pH 7.6 ± 0.2 at 25oC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure-121oC.

Use: For the differentiation of Gram-negative bacte-
ria of the intestinal groups, particularly members of the Salmonella (paratyphoid) group based on their ability to ferment tartrate.


Phenylalanine Agar

(Phenylalanine Deaminase Medium)

Composition per liter:

Agar .............................................................. ...12.0g

NaCl................................................................. ..5.0g

Yeast extract..................................................... ..3.0g

DL-Phenylalanine............................................... .2.0g

Na2HPO4........................................................... .1.0g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.


 

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure-121oC. Pour into sterile Petri dishes or leave in tubes.

Use: For the differentiation of enteric Gram-nega-
tive bacilli on the basis of their ability to produce
phenylpyruvic acid from phenylalanine. After appro-
priate incubation of bacteria, ferric chloride reagent
is added on the agar. Formation of a green color in 1-
5 min indicates the production of phenylpyruvic acid.


Phenylalanine Malonate Broth

 Composition per liter:

Sodium malonate ............................................. ...3.0g

DL-Phenylalanine ............................................. ...2.0g

NaCl.................................................................. ..2.0g

(NH4)2SO4 ........................................................ .2.0g

Yeast extract...................................................... ..1.0g

K2HPO4........................................................... ...0.6g

KH2PO4........................................................... ...0.4g

Bromthymol Blue .......................................... .0.025g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 10 min at 10 psi pressure-115oC.

Use: For the differentiation of Gram-negative enter-
ic bacilli on the basis of malonate utilization and for-
mation of pyruvic acid from phenylalanine.


Phenylethanol Agar

Composition per liter:

Agar .............................................................. ...15.0g

Tryptose ........................................................ ...10.0g

NaCl.................................................................. ..5.0g

Beef extract...................................................... ...3.0g

Phenylethanol ................................................... ..2.5g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure-121oC. Pour into sterile Petri dish-
es or leave in tubes.

Use: For the isolation of staphylococci and strepto-

cocci from specimens containing a mixed flora.


Phenylethanol Blood Agar

Composition per liter:

Agar .............................................................. ...15.0g

Tryptose ........................................................ ...10.0g

NaCl................................................................. ..5.0g

Beef extract...................................................... ...3.0g

Phenylethanol.................................................. ...2.5g

Blood, defibrinated ................................... ...50.0mL

pH 7.3 ± 0.2 at 25oC

Preparation of Medium: Add components, ex-
cept blood, to distilled/deionized water and bring
volume to 950.0mL. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 13 psi
pressure-118oC. Cool to 45o-50oC. Aseptically add
sterile defibrinated blood. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation of staphylococci and strepto-
cocci from specimens containing a mixed flora.


PHYG Medium

Composition per 110.1mL:

Beef heart, solids from infusion..................... ...10.0g

Polypeptone ..................................................... ..2.0g

Gelatin.............................................................. ..1.0g

Glucose ........................................................... ...1.0g

Yeast extract..................................................... ..1.0g

NaHCO3........................................................... ..0.5g

Tryptose ........................................................... ..0.2g

Agar .............................................................. ...0.16g

NaCl................................................................. ..0.1g

L-Cysteine*HCl*H2O...................................... ..0.09g

(NH4)2SO4..................................................... ...0.05g

Resazurin ..................................................... .0.16mg

Salts solution............................................... .50.0mL

Rabbit serum, inactivated ........................... ..10.0mL

Thiamine pyrophosphate solution.................. .0.1mL

pH 7.2-7.5 at 25oC

Salts Solution:

Composition per 400.0mL:

K2HPO4........................................................... ...0.9g

NaCl................................................................. ..0.8g

KH2PO4........................................................... ...0.4g

MnCl2*4H2O ................................................. ..0.16g

MgSO4 .......................................................... ..0.08g

Preparation of Salts Solution: Add components to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. 

Thiamine Pyrophosphate Solution:

Composition per 10.0mL:

Thiamine pyrophosphate ................................. .0.05g

Preparation of Thiamine Pyrophosphate So-
lution: Add thiamine pyrophosphate to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept rabbit serum and thiamine pyrophosphate solu-
tion, to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure-
121oC. Cool to 45o-50oC. Aseptically add 10.0mL
of sterile rabbit serum and 0.1mL of sterile thiamine
pyrophosphate solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of treponemes.


Pike Streptococcal Broth

Composit