Neisseria Medium

Composition per liter:

Biosate ........................................................... ..10.0g

Polypeptone .................................................. ...10.0g

NaCl................................................................. ..5.0g

Myosate........................................................... ...3.0g

Agar ................................................................. ..1.5g

Phenol Red.................................................. ...0.017g

Carbohydrate solution................................. ..50.0mL

pH 7.4-7.6 at 25oC

 

Carbohydrate Solution:

Composition per 50.0mL:

Carbohydrate................................................... ..10.0g

Preparation of Carbohydrate Solution: Add glucose, sucrose, or maltose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept carbohydrate solution, to distilled/deionized wa-
ter and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure-121oC. Cool to 45o-50oC.
Aseptically add sterile carbohydrate solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.

Use: For the cultivation of Neisseria species.


 

Neisseria meningitidis Medium

Composition per liter:

Beef infusion................................................ ...300.0g

Acid hydrolysate of casein.............................. ..17.5g

Agar .............................................................. ...17.0g

Starch............................................................... ...1.5g

Antibiotic solution ....................................... . 10.0mL

pH 7.4 ± 0.2 at 25oC

Antibiotic Solution:

Composition per 10.0mL:

Vancomycin .................................................. ..3.0mg

Colistin............................................................ .7.5mg

Nystatin...................................................... ..12,500U

Preparation of Antibiotic Solution: Add com-
ponents to distilled/deionized water and bring vol-
ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept antibiotic solution, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15
psi pressure-121oC. Cool to 45o-50oC. Aseptically
add sterile antibiotic solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of Neisseria meningitidis.


 

Nelson Culture Medium for Naegleria

 Composition per 100.0mL:

Glucose ........................................................... .0.17g

Panmede......................................................... ...0.17g

Na2HPO4..................................................... ..14.2mg

KH2PO4........................................................ .13.6mg

NaCl............................................................ ...12.0mg

CaCl2*2H2O .................................................. ..0.4mg

MgSO4*7H2O............................................... ...0.4mg

Bovine serum, heat-inactivated fetal .......... ... 10.0mL

 

Source: Panmede is available from Paines and By-

rne Ltd., Greenford, England, and Harrisons and Crosfield, Inc., Bronxville, NY.

Preparation of Medium: Add components, ex-
cept bovine serum, to distilled/deionized water and
bring volume to 90.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure-121oC. Cool to 25oC.
Aseptically add 10.0mL of sterile, heat-inactivated
fetal bovine serum. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or flasks. Use immediately.

Use: For the cultivation of Naegleria fowleri and
Paratetramitus jugosus.

 


 

Nelson Culture Medium for Naegleria

Composition per 100.0mL:

Glucose ........................................................... .0.17g

Liver infusion.................................................. .0.17g

Na2HPO4..................................................... ..14.2mg

KH2PO4........................................................ .13.6mg

NaCl........................................................... ...12.0mg

CaCl2*2H2O.................................................. ...0.4mg

MgSO4*7H2O............................................... ...0.4mg

Bovine serum, heat-inactivated fetal .......... ...10.0mL

Preparation of Medium: Add components, ex-
cept bovine serum, to distilled/deionized water and
bring volume to 90.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure-121oC. Cool to 25oC.
Aseptically add 10.0mL of sterile, heat-inactivated
fetal bovine serum. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or flasks. Use immediately.
Use: For the cultivation of Naegleria fowleri and
Paratetramitus jugosus.

 

Nelson Medium for Naegleria fowleri Composition per liter:

Glucose ........................................................... ...1.0g

Ox liver digest.................................................. ..1.0g

Page’s amoeba saline ...................................... .. 1.0L

Fetal calf serum, inactivated......................... .20.0mL

Page’s Amoeba Saline:

Composition per liter:

Na2HPO4..................................................... ...0.142g

KH2PO4........................................................ ..0.136g

NaCl.............................................................. ...0.12g

MgSO4*7H2O............................................... ...4.0mg

CaCl2*2H2O.................................................. ...4.0mg

Preparation of Page’s Amoeba Saline: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Preparation of Medium: Add the glucose and ox
liver digest to 1.0L of Page’s amoeba saline. Mix
thoroughly. Distribute into screw-capped tubes in

 

 

10.0mL volumes. Autoclave for 15 min at 15 psi pressure-121oC.  Cool  to 25oC.  Aseptically  add

0.2mL of sterile fetal calf serum to each tube. Mix thoroughly.

Use: For the cultivation of Naegleria fowleri.

 


 

Neomycin Agar, Modified

Composition per liter:

Agar .............................................................. ...15.0g

Peptone ........................................................... ...6.0g

Pancreatic digest of casein.................................. .4.0g

Yeast extract...................................................... ..3.0g

Beef extract...................................................... ...1.5g

Glucose ........................................................... ...1.0g

Methanol...................................................... . 20.0mL

Neomycin solution....................................... . 10.0mL

pH 7.0 ± 0.2 at 25oC

Neomycin Solution:

Composition per 10.0mL:

Neomycin sulfate............................................. ...1.0g

Preparation of Neomycin Solution: Add neo-
mycin sulfate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept methanol and neomycin solution, to distilled/
deionized water and bring volume to 970.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure-121oC. Cool to
45o-50oC. Filter sterilize methanol. To cooled, ster-
ile basal medium, aseptically add sterile methanol
and sterile neomycin solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Borde-
tella bronchiseptica.

 


 

Neomycin Blood Agar

Composition per liter:

Pancreatic digest of casein............................... ..14.5g

Agar .............................................................. ...14.0g

Papaic digest of soybean meal............................ .5.0g

NaCl.................................................................. ..5.0g

Growth factors .................................................. .1.5g

Sheep blood, defibrinated............................ .. 50.0mL

Neomycin solution....................................... . 10.0mL

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Neomycin Solution:

Composition per 10.0mL:

Neomycin sulfate............................................. .0.03g

 

Preparation of Neomycin Solution: Add neo-

mycin sulfate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept sheep blood and neomycin solution, to distilled/
deionized water and bring volume to 940.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure-121oC. Cool to
45o-50oC. Aseptically add sterile sheep blood and
sterile neomycin solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of group A
streptococci (Streptococcus pyogenes) and group B
streptococci (Streptococcus agalactiae) from throat
cultures and other clinical specimens.

 


 

Neomycin Luria Agar

Composition per liter:

Agar .............................................................. ...15.0g

Pancreatic digest of casein.............................. ..10.0g

Yeast extract..................................................... ..5.0g

NaCl................................................................. ..0.5g

Glucose solution ......................................... .20.0mL

Neomycin.................................................. ...10.0mL

pH 7.0 ± 0.1 at 25oC

Glucose Solution:

Composition per 100.0mL:

Glucose ........................................................... .10.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Neomycin Solution:

Composition per 10.0mL:

Neomycin sulfate.......................................... .12.0mg

Preparation of Neomycin Solution: Add neo-
mycin sulfate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glucose solution and neomycin solution, to distilled/ deionized water and bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121oC. Aseptically add 20.0mL of sterile glucose solution and 10.0mL of sterile neomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Escherichia coli.


 

Neomycin Medium No. 1

Composition per liter:

Agar .............................................................. ...15.0g

Peptone.............................................................. .5.0g

NaCl.................................................................. ..5.0g

Yeast extract...................................................... ..2.0g

Beef extract...................................................... ...1.0g

Sucrose solution.......................................... .. 20.0mL

Neomycin................................................... ... 10.0mL

pH 7.0 ± 0.1 at 25oC

Sucrose Solution:

Composition per 100.0mL:

Sucrose.............................................................. .2.5g

Preparation of Sucrose Solution: Add sucrose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Neomycin Solution:

Composition per 10.0mL:

Neomycin sulfate....................................... ..500.0mg

Preparation of Neomycin Solution: Add neo-
mycin sulfate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except sucrose solution and neomycin solution, to distilled/ deionized water and bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121oC. Aseptically add 20.0mL of sterile sucrose solution and 10.0mL of sterile neomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Pseudomonas aeruginosa.


 

Neopeptone Glucose Agar

Composition per liter:

Agar .............................................................. ...20.0g

Glucose ........................................................... .10.0g

Neopeptone...................................................... ...5.0g

pH 6.5 ± 0.2 at 25oC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC. Adjust pH to 6.5. Pour into sterile Petri dishes or leave in tubes.

Use: For the maintenance of stock cultures of a vari-
ety of microorganisms.

 


 

Neopeptone Glucose Rose Bengal

Aureomycinฎ Agar

Composition per liter:

Agar .............................................................. ...20.0g

Neopeptone...................................................... ...5.0g

Glucose ........................................................... ...1.0g

Tetracycline solution.................................... ...5.0mL

Rose Bengal solution ................................... ..3.5mL

pH 6.5 ± 0.2 at 25oC

Tetracycline Solution:

Composition per 150.0mL:

Tetracycline...................................................... ...1.0g

Preparation of Tetracycline Solution: Add tet-
racycline to distilled/deionized water and bring vol-
ume to 150.0mL. Mix thoroughly. Filter sterilize.

Rose Bengal Solution:

Composition per 100.0mL:

Rose Bengal...................................................... ..1.0g

Preparation of Rose Bengal Solution: Add
Rose Bengal to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex-
cept tetracycline solution, to distilled/deionized wa-
ter and bring volume to 995.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure-121oC. Cool to 45o-50oC.
Aseptically add 5.0mL of sterile tetracycline solu-
tion. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.

Use: For the isolation and cultivation of a wide vari-
ety of fungal species.

 


 

Neopeptone Infusion Agar

Composition per liter:

Beef heart, infusion from.............................. ..500.0g

Neopeptone...................................................... .20.0g

Agar .............................................................. ...20.0g

NaCl................................................................. ..5.0g

Sheep blood, defibrinated .......................... ..50.0mL

pH 7.4 ± 0.2 at 25oC

Preparation of Medium: Add components, ex-
cept sheep blood, to distilled/deionized water and
bring volume to 950.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15
psi pressure-121oC. Cool to 45o-50oC. Aseptically
add ster