Motility GI Medium

Composition per liter:

Gelatin............................................................ ...53.4g

Heart infusion broth....................................... ...25.0g

Agar ................................................................. ..3.0g

pH 7.2 ± 0.2 at 25oC

Source: This medium is available as a premixed

powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to cold distilled/deionized water and bring to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distrib-
ute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC. Pour into sterile Petri dishes in 20.0mL volumes or leave in tubes.

Use: For demonstrating the motility of microorgan-
isms and for separating organisms in their motile
phase.


Motility Indole Ornithine Medium

(MIO Medium)

Composition per liter:

Pancreatic digest of gelatin............................... .10.0g

Pancreatic digest of casein................................. .9.5g

L-Ornithine*HCl ............................................... ..5.0g

Yeast extract..................................................... ..3.0g

Agar ................................................................. ..2.0g

Glucose ........................................................... ...1.5g

Bromcresol Purple ......................................... ..0.02g

pH 6.6 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi
pressure-121oC.

Use:  For  the  differentiation  of  Gram-negative enteric bacteria based on their motility, indole pro-
duction, and ornithine decarboxylase activity.


Motility Medium S

Composition per liter:

Beef heart, solids from infusion..................... .500.0g

Gelatin........................................................... ...30.0g

Enzymatic hydrolyzate of protein................... ...10.0g

NaCl................................................................. ..5.0g

K2HPO4........................................................... ...2.0g

KNO3 .............................................................. ...2.0g

Agar ................................................................. ..1.0g

2,3,5-Triphenyltetrazolium

chloride solution.................................... .10.0mL

pH 7.2 ± 0.2 at 25oC

2,3,5-Triphenyltetrazolium  Chloride  Solu-
tion:

Composition per 10.0mL:

2,3,5-Triphenyltetrazolium chloride................... .0.1g

Preparation  of 2,3,5-Triphenyltetrazolium

Chloride Solution: Add 2,3,5-triphenyltetrazoli-

um chloride to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept 2,3,5-triphenyltetrazolium chloride solution, to
distilled/deionized  water  and  bring  volume  to
990.0mL. Mix thoroughly. Gently heat while stirring
and bring to boiling. Autoclave for 15 min at 15 psi
pressure-121oC.  Cool  to 60oC.  Aseptically  add

10.0mL  of  the  sterile 2,3,5-triphenyltetrazolium

chloride solution. Mix thoroughly. Aseptically dis-
tribute into sterile tubes. Keep at 4o-8oC until used.

Use: For the determination of bacterial motility.


Motility Nitrate Agar

Composition per liter:

Beef heart, solids from infusion..................... .100.0g

Tryptose ........................................................ ...12.0g

Agar ................................................................. ..3.0g

NaCl.................................................................. ..1.0g

KNO3 .............................................................. ...1.0g

Glucose ........................................................... ...0.5g

pH 7.4 ± 0.2 at 25oC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure-121oC.

Use: For the cultivation and observation of motility
and nitrate reduction in a variety of Gram-negative
bacteria.


Motility Nitrate Medium

(FDA M101)

Composition per liter:

Beef heart, solids from infusion..................... .100.0g

Tryptose ........................................................ ...12.0g

Agar ................................................................. ..3.0g

NaCl.................................................................. ..1.0g

KNO3, nitrite free ............................................. ..1.0g

pH 7.4 ± 0.2 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into screw-capped tubes in 4.0mL vol-
umes. Autoclave for 15 min at 15 psi pressure-
121oC.

Use: For the cultivation and differentiation of Gram-
negative nonfermentative bacteria from cosmetics based on their motility and their ability to reduce nitrate to nitrite.


Motility Nitrate Medium

Composition per liter:

Beef heart, solids from infusion..................... .100.0g

Tryptose ........................................................ ...12.0g

Agar ................................................................. ..3.0g

NaCl................................................................. ..1.0g

KNO3 .............................................................. ...1.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure-121oC.

Use: For the cultivation and observation of motility
and nitrate reduction in a variety of Gram-negative
nonfermentative bacteria isolated from cosmetics.


Motility Nitrate Medium, Buffered Composition per liter:

Peptone.............................................................. .5.0g

Galactose........................................................... .5.0g

Agar ................................................................. ..3.0g

Beef extract...................................................... ...3.0g

Na2HPO4........................................................... .2.5g

KNO3 .............................................................. ...1.0g

Glycerin ........................................................ .5.0mL

pH 7.3 ± 0.1 at 25oC

Preparation of Medium: Add components, ex-
cept agar, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Gently heat until dissolved. Add agar. Gently heat until boiling. Dis-
tribute into tubes in 11.0mL volumes. Autoclave for 15 min at 15 psi pressure-121oC. If not used within 4 hr, heat tubes to 100oC for 10 min.

Use: For the cultivation and observation of the motility of Clostridium perfringens.


Motility Sulfide Medium

Composition per liter:

Gelatin........................................................... ...80.0g

Proteose peptone............................................. ..10.0g

NaCl................................................................. ..5.0g

Agar ................................................................. ..4.0g

Beef extract...................................................... ...3.0g

Sodium citrate................................................... ..2.0g

L-Cystine ........................................................... .0.2g

Ferrous ammonium citrate................................. .0.2g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes in 4-5.0mL volumes. Autoclave for 15 min at 10 psi pressure-116oC.

Use: For the determination of bacterial motility and
the ability of bacteria to produce H2S from L-cystine.
For the differentiation of Gram-negative bacteria of

the Enterobacteriaceae.


Motility Test and Maintenance Medium Composition per liter:

Peptone ........................................................... .10.0g

NaCl................................................................. ..5.0g

Agar ................................................................. ..4.0g

Beef extract...................................................... ...3.0g

2,3,5-Triphenyltetrazolium chloride................ ..0.05g

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into screw-capped tubes

in 8.0mL volumes. Autoclave for 15 min at 15 psi pressure-121oC.

Use: For the cultivation, maintenance, and observa-
tion of the motility of Listeria monocytogenes.


Motility Test and

Maintenance Medium

Composition per liter:

Agar ................................................................. ..9.0g

Tryptose ........................................................... ..8.0g

NaCl................................................................. ..5.0g

Pancreatic digest of gelatin............................... ...2.5g

Beef extract...................................................... ...1.5g

pH 7.2 ± 0.1 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes in 7.0mL volumes. Autoclave
for 15 min at 15 psi pressure-121oC. Cool to 45o-
50oC. Pass the cooled tubes into an anaerobic cham-

ber containing 85% N2 + 10% H2 + 5% CO2.

Use: For the cultivation, maintenance, and observa-
tion of the motility in a variety of anaerobic bacteria.


Motility Test and

Maintenance Medium

Composition per liter:

Peptone ........................................................... .10.0g

NaCl................................................................. ..5.0g

Agar ................................................................. ..4.0g

Beef extract...................................................... ...3.0g

pH 7.4 ± 0.1 at 25oC

Preparation of Medium: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Distribute into screw-capped tubes in 8.0mL

volumes. Autoclave for 15 min at 15 psi pressure-
121oC.

Use: For the cultivation, maintenance, and observa-
tion of motility in members of the Enterobacteriaceae.


Motility Test and

Maintenance Medium, Gilardi

Composition per liter:

Pancreatic digest of casein.............................. ..10.0g

NaCl................................................................. ..5.0g

Agar ................................................................. ..3.0g

Yeast extract..................................................... ..3.0g

pH 7.2 ± 0.1 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into screw-capped tubes
in 3.5mL volumes. Autoclave for 15 min at 15 psi
pressure-121oC.

Use: For the cultivation, maintenance, and observa-
tion of motility in nonfermenting Gram-negative
bacteria.


Motility Test and

Maintenance Medium, Tatum

Composition per liter:

Tryptose ........................................................... ..8.0g

NaCl................................................................. ..5.0g

Agar ................................................................. ..4.0g

Pancreatic digest of gelatin............................... ...2.5g

Beef extract...................................................... ...1.5g

pH 6.9 ± 0.2 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into screw-capped tubes
in 8.0mL volumes. Autoclave for 15 min at 15 psi
pressure-121oC.

Use: For the cultivation, maintenance, and observa-
tion of motility in nonfermenting Gram-negative
bacteria.


Motility Test Medium

Composition per liter:

Pancreatic digest of gelatin............................... .10.0g

NaCl................................................................. ..5.0g

Agar ................................................................. ..4.0g

Beef extract...................................................... ...3.0g

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

2,3,5-Triphenyltetrazolium  Chloride  Solu-

tion:

Composition per 10.0mL:

2,3,5-Triphenyltetrazolium chloride................... .0.1g

Preparation  of 2,3,5-Triphenyltetrazolium

Chloride Solution: Add 2,3,5-triphenyltetrazoli-
um chloride to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to
distilled/deionized  water  and  bring  volume  to
995.0mL. Mix thoroughly. Gently heat while stirring
and bring to boiling. Autoclave for 15 min at 15 psi
pressure-121oC. Cool to 45o-50oC. Aseptically add
5.0mL of sterile 2,3,5-triphenyltetrazolium chloride
solution. Mix thoroughly. Aseptically distribute into
sterile tubes.