รวมอาหารเลี้ยงเชื้อหมวด M

posted on 04 Jun 2012 22:23 by moomsabuy in medium directory Knowledge

M7 Medium

Composition per liter:

Yeast extract solution................................. . 200.0mL

Dialyzed fetal bovine serum...................... .. 100.0mL

L-Methionine solution................................. .. 30.0mL

Buffer solution............................................. . 20.0mL

Glucose solution ......................................... . 20.0mL

Yeast Extract Solution:

Composition per liter:

Yeast extract................................................... ...25.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121oC. Cool to 25oC.

L-Methionine Solution:

Composition per liter:

L-Methionine...................................................... .1.5g

Preparation of L-Methionine Solution: Add L-
methionine to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121oC. Cool to 25oC.

Glucose Solution:

Composition per liter:

Glucose ........................................................ ..270.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121oC. Cool to 25oC.

Buffer Solution:

Composition per liter:

Na2HPO4........................................................... 25.0g

KH2PO4........................................................... .18.1g

Preparation of Buffer Solution: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121oC. Cool to 25oC.

Dialyzed Fetal Bovine Serum:

Composition per 100.0mL:

Fetal bovine serum, heat inactivated .......... . 100.0mL

Preparation of Dialyzed Fetal Bovine Se-
rum: Dialyze the heat-inactivated serum at 0o-4oC
against 10 volumes of water. Clean the dialysis tubing
before use by boiling in a solution of 0.37g/L of EDTA
and rinsing with water. Change the water four times at

8-16 hr intervals. Centrifuge the dialyzed serum for 30

min at 35,000 x g. Filter sterilize serum fluid.

Preparation of Medium: Aseptically combine the sterile solutions. Mix thoroughly. Bring volume to 1.0L with sterile distilled/deionized water.

Use: For the cultivation of Naegleria fowleri, Nae-
gleria gruberi, and Nuclearia species.


M9 Medium

with Casamino Acids

Composition per liter:

Na2HPO4........................................................... .6.0g

Casamino acids ............................................... ...5.0g

KH2PO4........................................................... ...3.0g

NH4Cl .............................................................. ..1.0g

NaCl................................................................. ..0.5g

Glucose solution ......................................... .10.0mL

MgSO4*7H2O solution ............................... ...1.0mL

Thiamine*HCl solution................................ ...1.0mL

CaCl2 solution................................................ .1.0mL

pH 7.0 ± 0.2 at 25oC

Glucose solution:

Composition per 100.0mL:

D-Glucose........................................................ .20.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121oC.

MgSO4*7H2O Solution:

Composition per liter:

MgSO4*7H2O............................................... ..246.5g

Preparation of MgSO4*7H2O Solution: Add MgSO4*7H2O to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121oC.

Thiamine*HCl Solution:

Composition per 10.0mL:

Thiamine*HCl ............................................ ...10.0mg

Preparation of Thiamine*HCl Solution: Add thiamine*HCl to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

CaCl2 Solution:

Composition per liter:

CaCl2 solution................................................ ...14.7g

Preparation of CaCl2 Solution: Add CaCl2 so-
lution to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121oC.

Preparation of Medium: Add components, ex-
cept MgSO4*7H2O solution, glucose solution, thia-

mine*HCl solution, and CaCl2 solution, to distilled/
deionized water and bring volume to 987.0mL. Mix
thoroughly. Adjust pH to 7.0. Autoclave for 15 min
at 15 psi pressure-121oC. Cool to room temperature.
Aseptically add sterile MgSO4*7H2O solution, sterile
glucose solution, sterile thiamine*HCl solution, and
sterile CaCl2 solution. Mix thoroughly. Distribute
into tubes or flasks.

Use: For the cultivation and maintenance of Fla-
vobacterium meningosepticum.


MacConkey Agar

Composition per liter:

Pancreatic digest of gelatin............................... .17.0g

Agar .............................................................. ...13.5g

Lactose............................................................ ..10.0g

NaCl.................................................................. ..5.0g

Bile salts............................................................ ..1.5g

Pancreatic digest of casein.................................. .1.5g

Peptic digest of animal tissue ........................... ..1.5g

Neutral Red...................................................... .0.03g

Crystal Violet................................................ ...1.0mg

pH 7.1 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil-
ing. Autoclave for 15 min at 15 psi pressure-121oC.
Pour into sterile Petri dishes or distribute into sterile
tubes.

Use: For the selective isolation, cultivation, and dif-
ferentiation of coliforms and enteric pathogens based
on the ability to ferment lactose. Lactose-fermenting
organisms appear as red to pink colonies. Lactose-
nonfermenting organisms appear as colorless or
transparent colonies.


MacConkey Agar

Composition per liter:

Peptone ........................................................... .20.0g

Agar .............................................................. ...12.0g

Lactose............................................................ ..10.0g

Bile salts............................................................ ..5.0g

NaCl.................................................................. ..5.0g

Neutral Red................................................... ..0.075g

pH 7.4 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil-

ing. Autoclave for 15 min at 15 psi pressure-121oC.

Pour into sterile Petri dishes or distribute into sterile
tubes.

Use: For the selective isolation, cultivation, and dif-
ferentiation of coliforms and enteric pathogens based
on the ability to ferment lactose. Lactose-fermenting
organisms appear as red to pink colonies. Lactose-
nonfermenting organisms appear as colorless or
transparent colonies.


MacConkey Agar, CS

Composition per liter:

Peptone........................................................... ..17.0g

Agar .............................................................. ...13.5g

Lactose............................................................ ..10.0g

NaCl................................................................. ..5.0g

Proteose peptone................................................ .3.0g

Bile salts........................................................... ..1.5g

Neutral Red...................................................... .0.03g

Crystal Violet................................................ ...1.0mg

pH 7.1 ± 0.2 at 25oC

Source: This medium is available as a prepared me-
dium from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil-
ing. Autoclave for 15 min at 15 psi pressure-121oC.
Pour into sterile Petri dishes or distribute into sterile
tubes.

Use: For the cultivation and differentiation of lac-
tose-fermenting and nonfermenting Gram-negative
bacteria while also controlling the swarming of Pro-
teus species, if present. Lactose-fermenting organ-
isms appear as red to pink colonies. Lactose-nonfer-
menting organisms appear as colorless or transparent
colonies.


MacConkey Agar No. 2

(MacConkey II Agar)

Composition per liter:

Peptone........................................................... ..20.0g

Agar .............................................................. ...15.0g

Lactose............................................................ ..10.0g

NaCl................................................................. ..5.0g

Bile salts no. 2.................................................. ..1.5g

Neutral Red...................................................... .0.05g

Crystal Violet................................................ ...1.0mg

pH 7.2 ± 0.2 at 25oC

Source: This medium is available as a premixed
powder from  BD Diagnostic Systems and Oxoid Un-
ipath.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil-
ing. Autoclave for 15 min at 15 psi pressure-121oC.
Pour into sterile Petri dishes or distribute into sterile
tubes.

Use: For the selective isolation, cultivation, and dif-
ferentiation of enteric pathogens, especially entero-
cocci, in clinical specimens and in materials of sani-
tary importance.


MacConkey Agar No. 3

Composition per liter:

Peptone ........................................................... .20.0g

Agar .............................................................. ...15.0g

Lactose............................................................ ..10.0g

NaCl.................................................................. ..5.0g

Bile salts no. 3................................................... ..1.5g

Neutral Red...................................................... .0.03g

Crystal Violet................................................ ..0.001g

pH 7.1 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil-
ing. Autoclave for 15 min at 15 psi pressure-121oC.
Pour into sterile Petri dishes or distribute into sterile
tubes.

Use: For the selective isolation, cultivation, and dif-
ferentiation of enteric pathogens, especially Salmo-
nella and Shigella, in clinical specimens.


MacConkey Agar without Crystal Violet

Composition per liter:

Agar .............................................................. ...12.0g

Lactose............................................................ ..10.0g

Pancreatic digest of casein............................... ..10.0g

Peptic digest of animal tissue ........................ ...10.0g

Bile salts............................................................ ..5.0g

NaCl.................................................................. ..5.0g

Neutral Red...................................................... .0.05g

pH 7.4 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil-
ing. Autoclave for 15 min at 15 psi pressure-121oC.
Pour into sterile Petri dishes or distribute into sterile
tubes.

Use: For the detection of members of the Enterobac-

teriaceae and enterococci as well as some staphylo-
cocci.


MacConkey Agar, Fluorocult

(Fluorocult MacConkey Agar)

Composition per liter:

Peptone from casein....................................... ...17.0g

Agar .............................................................. ...13.5g

Lactose............................................................ ..10.0g

NaCl................................................................. ..5.0g

Peptone from meat............................................. .3.0g

Bile salt mixture................................................ ..1.5g

4-Methylumbelliferyl-β-D-glucuronide............. ..0.1g

Neutral Red...................................................... .0.03g

Crystal Violet................................................ ..0.001g

pH 7.1 ± 0.2 at 25oC

Source: This medium is available from Merck.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Autoclave for 15 min at 15 psi pres-
sure-121oC. Cool to 45o-50oC. Pour into sterile Petri
dishes. The plates are clear and red to red-brown.

Use: For the isolation of Salmonella, Shigella, and
coliform bacteria, in particular E. coli, from various
specimens. The bile salts and crystal violet largely in-
hibit the growth of Gram-positive microbial flora.
Lactose together with the pH indicator neutral red are
used to detect lactose-positive colonies and E. coli
can be seen among these because of fluorescence un-
der UV light.


MacConkey Agar without Salt

Composition per liter:

Peptone........................................................... ..20.0g

Agar .............................................................. ...12.0g

Lactose............................................................ ..10.0g

Bile salts........................................................... ..5.0g

Neutral Red................................................... ..0.075g

pH 7.4 ± 0.2 at 25oC

Source: This medium is available as a premixed
powder from  BD Diagnostic Systems and Oxoid Un-
ipath.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil-
ing. Autoclave for 15 min at 15 psi pressure-121oC.
Pour into sterile Petri dishes or distribute into sterile
tubes. Dry the surface of plates before inoculation.

Use: For the isolation and detection of coliforms and
enteric pathogens from urine. Provides a low electro-

lyte medium on which most Proteus species will not
swarm and therefore avoids overgrowth of the plate.


MacConkey MUG Agar

Composition per liter:

Casein peptone................................................ ..17.0g

Agar .............................................................. ...13.5g

Lactose............................................................ ..10.0g

NaCl................................................................. ..5.0g

Meat peptone .................................................. ...3.0g

Bile salt mixture................................................ ..1.5g

4-Methylumbelliferyl-β-D-glucuronide............. ..0.1g

Neutral Red...................................................... .0.03g

Crystal Violet................................................ ..0.001g

pH 7.1 ± 0.2 at 37oC

Source: This medium is available from Fluka, Sig-
ma-Aldrich.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring
to boiling. Autoclave for 15 min at 15 psi pressure-
121oC. Cool to 50oC. Pour into sterile Petri dishes.
Use: For the isolation of Salmonella, Shigella, and
coliform bacteria, in particular E. coli, from diverse
specimens. Bile salts and crystal violet extensively
inhibit the Gram-positive flora. The presence of lac-
tose and neutral red indicate lactose-positive colonies
from which E. coli can be identified by fluorescence
in the UV.


Magnesium Oxalate Agar

(MOX Agar)

Composition per liter:

Pancreatic digest of casein.............................. ..15.0g

Agar .............................................................. ...15.0g

Papaic digest of soybean meal........................... .5.0g

NaCl................................................................. ..5.0g

MgCl2*6H2O .................................................... .4.1g

Sodium oxalate ............................................... .2.68g

pH 7.4-7.6 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure-121oC. Pour into sterile Petri dish-
es or leave in tubes.

Use: For the cultivation of Yersinia enterocolitica.


Maintenance of L Antigen

in Neisseria

Composition per liter:

Proteose peptone no. 3.................................... ..20.0g

Agar .............................................................. ...15.0g

Na2HPO4........................................................... .5.0g

NaCl................................................................. ..5.0g

Glucose ........................................................... ...0.5g

Rabbit blood, defibrinated ........................ ..100.0mL

pH 7.4-7.6 at 25oC

Preparation of Medium: Add components, ex-
cept rabbit blood, to distilled/deionized water and
bring volume to 900.0mL. Mix thoroughly. Gently
heat while stirring until boiling. Autoclave for 20 min
at 15 psi pressure-121oC. Cool to 60oC. Aseptically
add 100.0mL of sterile, defibrinated rabbit blood.
Maintain at 75oC while shaking for 30 min. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Neis-
seria gonorrhoeae.


Malachite Green Broth

Composition per liter:

Peptone........................................................... ..15.0g

Beef extract...................................................... ...9.0g

Malachite Green.......................................... ...0.01mg

pH 7.3 ± 0.2 at 25oC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure-121oC.

Use: For the cultivation of Pseudomonas aeruginosa.


Malonate Broth

Composition per liter:

Sodium malonate ............................................ ...3.0g

NaCl................................................................. ..2.0g

(NH4)2SO4........................................................ ..2.0g

K2HPO4........................................................... ...0.6g

KH2PO4........................................................... ...0.4g

Bromthymol Blue ......................................... .0.025g

pH 6.7 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure-121oC. Avoid in-
troduction of carbon and nitrogen from other sources.
Use:  For  the  cultivation  and  differentiation  of
coliforms and other enteric organisms, particularly
Enterobacter and Escherichia, based on their ability
to utilize malonate as the sole carbon source and
ammonium sulfate as the sole nitrogen source. Mal-
onate-utilizing organisms turn the medium blue.


Malonate Broth, Ewing Modified

Composition per liter:

Sodium malonate ............................................. ...3.0g

NaCl.................................................................. ..2.0g

(NH4)2SO4 ........................................................ .2.0g

Yeast extract...................................................... ..1.0g

K2HPO4........................................................... ...0.6g

KH2PO4........................................................... ...0.4g

Glucose ........................................................... .0.25g

Bromthymol Blue .......................................... .0.025g

pH 6.7 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure-121oC.

Use:  For  the  cultivation  and  differentiation  of
coliforms and other enteric organisms, particularly
Enterobacter and Escherichia, based on their ability
to utilize malonate as a carbon source and ammo-
nium sulfate as a nitrogen source. The small amount
of yeast extract and glucose encourages the growth of
some organisms that may be distressed or fail to
respond.  Malonate-utilizing  organisms  turn  the
medium blue.


Malt Agar

Composition per liter:

Agar .............................................................. ...20.0g

Malt extract...................................................... .12.5g

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat until boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi
pressure-121oC. Pour into sterile Petri dishes or
leave in tubes.

Use: For the cultivation and maintenance of fungi.


Mannitol Salt Agar

Composition per liter:

NaCl............................................................... ...75.0g

Agar .............................................................. ...15.0g

D-Mannitol ..................................................... ..10.0g

Pancreatic digest of casein.................................. .5.0g

Peptic digest of animal tissue ........................... ..5.0g

Beef extract...................................................... ...1.0g

Phenol Red................................................... ...0.025g

pH 7.4 ± 0.2 at 25oC

Source: This medium is available as a premixed
powder from  BD Diagnostic Systems and Oxoid Un-
ipath.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC. Pour into sterile Petri dishes or leave in tubes.

Use: For the selective isolation, cultivation, and enu-
meration of staphylococci from clinical and nonclin-
ical specimens. Mannitol-utilizing organisms turn the medium yellow.


Martin-Lewis Agar

Composition per liter:

Agar .............................................................. ...12.0g

Hemoglobin .................................................. ...10.0g

Pancreatic digest of casein................................. .7.5g

Selected meat peptone ....................................... .7.5g

NaCl................................................................. ..5.0g

K2HPO4........................................................... ...4.0g

Cornstarch........................................................ ..1.0g

KH2PO4........................................................... ...1.0g

Supplement solution ................................... .10.0mL

VCAT inhibitor ........................................ ...10.0mL

pH 7.2 ± 0.22 at 25oC

Source: Martin-Lewis agar is available as a pre-
pared medium from  BD Diagnostic Systems.

Supplement Solution:

Composition per liter:

Glucose ........................................................ ..100.0g

L-Cysteine*HCl............................................... ..25.9g

L-Glutamine..................................................... .10.0g

L-Cystine ........................................................... .1.1g

Adenine........................................................... ...1.0g

Nicotinamide adenine dinucleotide ................ ...0.25g

Vitamin B12 ..................................................... ...0.1g

Thiamine pyrophosphate.................................... .0.1g

Guanine*HCl ................................................. ..0.03g

Fe(NO3)3*6H2O............................................... .0.02g

p-Aminobenzoic acid.................................... ..0.013g

Thiamine*HCl ............................................... ..3.0mg

Source: The supplement solution IsoVitaleX en-
richment is available from BD Diagnostic Systems. This enrichment may be replaced by supplement VX from  BD Diagnostic Systems.

Preparation of Supplement Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

VCAT Inhibitor:

Composition per 10.0mL:

Colistin........................................................... .7.5mg

Trimethoprim lactate....................................... .5.0mg

Vancomycin .................................................. ..4.0mg

Anisomycin..................................................... .0.02g

Preparation of VCAT Inhibitor: Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept supplement solution enrichment and VCAT in-
hibitor, to distilled/deionized water and bring volume
to 980.0mL. Gently heat while stirring and bring to
boiling. Autoclave for 15 min at 15 psi pressure-
121oC. Cool to 45o-50oC. Aseptically add sterile
supplement solution enrichment and sterile VCAT
inhibitor. Mix thoroughly. Pour into sterile Petri dish-
es.

Use: For the isolation and cultivation of pathogenic Neisseria from specimens containing mixed flora of bacteria and fungi.


Martin-Lewis Agar, Enriched

Composition per liter:

Agar .............................................................. ...12.0g

Pancreatic digest of casein.................................. .7.5g

Selected meat peptone ....................................... .7.5g

NaCl.................................................................. ..5.0g

K2HPO4........................................................... ...4.0g

Cornstarch......................................................... ..1.0g

KH2PO4........................................................... ...1.0g

Sarcina lutea suspension ....................... ... 20.0mL

Horse serum, inactivated ........................... ... 20.0mL

Supplement solution .................................... . 10.0mL

PCAT inhibitor............................................ . 10.0mL

pH 7.2 ± 0.22 at 25oC

Source: The supplement solution (IsoVitaleX en-
richment) is available from BD Diagnostic Systems. This enrichment may be replaced by supplement VX from  BD Diagnostic Systems.

Sarcina lutea Suspension:

Composition per 20.0mL:

Sarcina lutea FDA 1001.................. ..106-107 cells

Preparation of Sarcina lutea Suspension:
Aseptically wash the growth of 24-h cultures of
Sarcina lutea FDA 1001 cells from Thayer-Martin
plates with sterile soybean casein digest broth. Stan-
dardize the suspension by adding additional sterile
tryptic soy broth to yield 40% light transmission at
530nm wavelength.

Soybean Casein Digest Broth:

Composition per liter:

Pancreatic digest of casein............................... ..17.0g

NaCl.................................................................. ..5.0g

Papaic digest of soybean meal............................ .3.0g

K2HPO4........................................................... ...2.5g

Glucose ........................................................... ...2.5g

pH 7.3 ± 0.2 at 25oC

Preparation of Soybean Casein Digest Broth:

Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC.

Supplement Solution:

Composition per liter:

Glucose ........................................................ ..100.0g

L-Cysteine*HCl............................................... ..25.9g

L-Glutamine..................................................... .10.0g

L-Cystine ........................................................... .1.1g

Adenine........................................................... ...1.0g

Nicotinamide adenine dinucleotide ................ ...0.25g

Vitamin B12 ..................................................... ...0.1g

Thiamine pyrophosphate.................................... .0.1g

Guanine*HCl ................................................. ..0.03g

Fe(NO3)3*6H2O............................................... .0.02g

p-Aminobenzoic acid.................................... ..0.013g

Thiamine*HCl ............................................... ..3.0mg

Preparation of Supplement Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

PCAT Inhibitor:

Composition per 10.0mL:

Anisomycin..................................................... .0.02g

Colistin........................................................... .7.5mg

Trimethoprim lactate....................................... .5.0mg

Penicillin G ............................................... ..25,000U

Preparation of PCAT Inhibitor: Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex-
cept Sarcina lutea suspension, horse serum, supple-
ment  solution,  and  PCAT  inhibitor—to  distilled/
deionized water and bring volume to 940.0mL. Gently
heat while stirring and bring to boiling. Autoclave for
15 min at 15 psi pressure-121oC. Cool to 45o-50oC.
Aseptically add 20.0mL of sterile Sarcina lutea sus-
pension, 20.0mL of sterile horse serum, 10.0mL of
supplement solution, and 10.0mL of sterile PCAT in-
hibitor. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the isolation and cultivation of pathogenic
Neisseria, especially penicillinase-producing strains,
from specimens containing mixed flora of bacteria
and fungi.


Maximum Recovery Diluent

Composition per liter:

NaCl................................................................. ..8.5g

Peptone.............................................................. .1.0g

pH 7.0 ± 0.2 at 25oC

Source: This medium is available as a premixed

powder from Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure-121oC.

Use: This diluent is a physiologically isotonic and protective medium for maximal recovery of microor-
ganisms from a variety of sources.


McBride Listeria Agar

Composition per liter:

Agar .............................................................. ...15.0g

Glycine............................................................ ..10.0g

Pancreatic digest of casein.................................. .5.0g

Peptic digest of animal tissue ........................... ..5.0g

NaCl.................................................................. ..5.0g

Beef extract...................................................... ...3.0g

Phenylethyl alcohol .......................................... ..2.5g

LiCl.................................................................. ...0.5g

pH 7.3 ± 0.22 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC. Pour into sterile Petri dishes or leave in tubes.

Use: For the selective isolation of Listeria monocy-
togenes from clinical and nonclinical specimens con-
taining mixed flora.


McCarthy Agar

Composition per liter:

Cornstarch...................................................... ...10.0g

Naladixic acid................................................. .0.015g

Colistin............................................................ ..0.01g

GC agar base................................................... ... 1.0L

pH 7.2 ± 0.2 at 25oC

GC Agar Base:

Composition per liter:

Agar .............................................................. ...10.0g

Pancreatic digest of casein.................................. .7.5g

Peptic digest of animal tissue ........................... ..7.5g

NaCl.................................................................. ..5.0g

K2HPO4........................................................... ...4.0g

Cornstarch......................................................... ..1.0g

KH2PO4........................................................... ...1.0g

Preparation of GC Agar Base: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: To 1.0L of GC agar

base, add the cornstarch. Gently heat while stirring to dissolve. Add the naladixic acid and colistin. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121oC. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and differentiation of Gard-
nerella vaginalis (Haemophilus vaginalis, Coryne-
bacterium vaginale) from genitourinary specimens. Bacteria that can utilize starch appear as colonies sur-
rounded by a clear zone.