LPM Agar

(Lithium Chloride Phenylethanol

Moxalactam Plating Agar)

Composition per liter:

Agar .............................................................. ...15.0g

Glycine anhydride.......................................... ...10.0g

LiCl.................................................................. ...5.0g

NaCl.................................................................. ..5.0g

Pancreatic digest of casein.................................. .5.0g

Peptic digest of animal tissue ........................... ..5.0g

Beef extract...................................................... ...3.0g

Phenylethyl alcohol .......................................... ..2.5g

Moxalactam solution .................................... .. 2.0mL

pH 7.3 ± 0.2 at 25oC

Source: This medium is available as a premixed

powder from  BD Diagnostic Systems.

Moxalactam Solution:

Composition per 10.0mL:

Moxalactam ..................................................... ..0.1g

Preparation of Moxalactam Solution: Add
moxalactam to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept moxalactam solution, to distilled/deionized wa-
ter and bring volume to 998.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Auto-
clave for 12 min at 15 psi pressure-121oC. Cool to 45o-50oC. Aseptically add 2.0mL of sterile moxalac-
tam solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Listeria monocytogenes.


LPM Agar with

Esculin and Ferric Iron

Composition per liter:

Agar .............................................................. ...15.0g

Glycine anhydride.......................................... ...10.0g

LiCl.................................................................. ...5.0g

NaCl................................................................. ..5.0g

Pancreatic digest of casein................................. .5.0g

Peptic digest of animal tissue........................... ...5.0g

Beef extract...................................................... ...3.0g

Phenylethyl alcohol.......................................... ...2.5g

Esculin .............................................................. .1.0g

Ferric ammonium citrate.................................... .0.5g

Moxalactam solution.................................... ...2.0mL

pH 7.3 ± 0.2 at 25oC

Moxalactam Solution:

Composition per 10.0mL:

Moxalactam ..................................................... ..0.1g

Preparation of Moxalactam Solution: Add
moxalactam to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex-
cept moxalactam solution, to distilled/deionized wa-
ter and bring volume to 998.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Auto-
clave for 12 min at 15 psi pressure-121oC. Cool to 45o-50oC. Aseptically add 2.0mL of sterile moxalac-
tam solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Listeria monocytogenes.


LST-MUG Broth

Composition per liter:

Tryptose ........................................................ ...20.0g

Lactose............................................................... .5.0g

NaCl.................................................................. ..5.0g

K2HPO4 ........................................................ ...2.75g

KH2PO4 ........................................................ ...2.75g

L-Tryptophan .................................................. ...1.0g

Sodium lauryl sulfate....................................... ...0.1g

4-Methylumbelliferyl-β-D-glucuronide............. ..0.1g

pH 6.8 ± 0.2 at 37oC

Source: This medium is available from Fluka, Sig-
ma-Aldrich.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into test tubes that con-
tain an inverted Durham tube in 10.0mL volumes.
Autoclave for 15 min at 15 psi pressure-121oC.

Use: For the detection of E. coli by the fluorgenic method. The presence of E. coli results in fluores-
cence in the UV. A positive indole test provide con-
firmation. β-D-glucoronidase, which is produced by E.  coli,  cleaves 4-methylumbelliferyl-β-D-glucu-

ronide to 4-methylumbelliferone and glucuronide. The fluorogen 4-methylumbelliferone can be detect-
ed under a long wavelength UV lamp.


Lysine Arginine Iron Agar

Composition per liter:

Agar .............................................................. ...15.0g

L-Arginine ..................................................... ...10.0g

L-Lysine........................................................... .10.0g

Peptone ........................................................... ...5.0g

Yeast extract...................................................... ..3.0g

Glucose ........................................................... ...1.0g

Ferric ammonium citrate..................................... .0.5g

Sodium thiosulfate.......................................... ..0.04g

Bromcresol Purple ......................................... ..0.02g

pH 6.8 ± 0.2 at 25oC

Preparation of Medium: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH
to 6.8. Distribute into screw-capped tubes in 5.0mL
volumes. Autoclave for 12 min at 15 psi pressure-
121oC. Allow tubes to cool in a slanted position.

Use: For the cultivation and differentiation of bacteria
based on their ability to decarboxylate lysine, decarbox-
ylate arginine, and produce H2S. Bacteria that decar-
boxylate lysine or arginine turn the medium purple.
Bacteria that produce H2S appear as black colonies.


Lysine Broth Falkow with NaCl

Composition per liter:

L-Lysine ........................................................... ..5.0g

Peptone or gelysate.......................................... ...5.0g

Yeast extract..................................................... ..3.0g

Glucose ........................................................... ...1.0g

Bromcresol Purple ......................................... ..0.02g

pH 6.5 ± 0.2 at 25oC

Preparation of Medium: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH so that it will be 6.5 ± 0.2 after sterilization. Distribute into 16 ื 150mm screw-

capped tubes in 5.0mL volumes. Autoclave medium
with loosely capped tubes for 10 min at 15 psi pressure-
121oC. Screw the caps on tightly for storage and after
inoculation.

Use: For the cultivation and differentiation of Vibrio spp. based on their ability to decarboxylate the amino acid lysine. Bacteria that decarboxylate lysine turn the medium turbid purple.


Lysine Decarboxylase Broth, Falkow Composition per liter:

Peptone.............................................................. .5.0g

L-Lysine........................................................... ...5.0g

Yeast extract..................................................... ..3.0g

Glucose ........................................................... ...1.0g

Bromcresol Purple ......................................... ..0.02g

pH 6.5-6.8 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Adjust pH to 6.5-6.8. Distribute into tubes in 5.0mL
volumes. Autoclave for 15 min at 15 psi pressure-
121oC.

Use: For the cultivation and differentiation of bacte-
ria, especially Salmonella, based on their ability to decarboxylate lysine. Bacteria that decarboxylate lysine turn the medium turbid purple.


Lysine Decarboxylase Broth,

Taylor Modification

Composition per liter:

L-Lysine........................................................... ...5.0g

Yeast extract..................................................... ..3.0g

Glucose ........................................................... ...1.0g

Bromcresol Purple ......................................... ..0.02g

pH 6.1 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.
Adjust pH to 6.1. Distribute into tubes in 5.0mL vol-
umes. Autoclave for 15 min at 15 psi pressure-
121oC.

Use: For the cultivation and differentiation of bacte-
ria, especially Salmonella, based on their ability to decarboxylate lysine. Bacteria that decarboxylate lysine turn the medium turbid purple.


Lysine Decarboxylase Broth,

Taylor Modification

(Lysine Decarboxylase Broth) Composition per liter:

L-Lysine........................................................... ...5.0g

Peptone ........................................................... ...5.0g

Yeast extract...................................................... ..3.0g

Glucose ........................................................... ...1.0g

Bromcresol Purple ......................................... ..0.02g

pH 6.8 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Adjust pH to 6.1. Distribute into tubes in 5.0mL vol-
umes. Autoclave for 15 min at 15 psi pressure-
121oC.

Use: For the cultivation and differentiation of bacte-
ria, especially Salmonella, based on their ability to decarboxylate lysine. Bacteria that decarboxylate lysine turn the medium turbid purple.

Lysine Decarboxylase Medium Composition per liter:

Glucose ........................................................... ...0.5g

KH2PO4........................................................... ...0.5g

L-Lysine*HCl..................................................... .0.5g

pH 4.6 ± 0.2 at 25oC

Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Adjust pH to 4.6. Autoclave for 15 min at 15 psi pres-
sure-121oC. Aseptically distribute into sterile tubes
in 1.0mL volumes.

Use: For the cultivation and differentiation of Gram-
negative, nonfermentative bacteria based on their ability to decarboxylate lysine. Bacteria that decar-
boxylate lysine turn the medium turbid purple.

Lysine Iron Agar

Composition per liter:

Agar .............................................................. ...13.5g

L-Lysine........................................................... .10.0g

Pancreatic digest of gelatin............................... ...5.0g

Yeast extract..................................................... ..3.0g

Glucose ........................................................... ...1.0g

Ferric ammonium citrate.................................... .0.5g

Na2S2O3*5H2O .............................................. ..0.04g

Bromcresol Purple ......................................... ..0.02g

pH 6.7 ± 0.2 at 25oC

Source: This medium is available as a premixed
powder from  BD Diagnostic Systems and Oxoid Un-
ipath.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 12 min at 15 psi pressure-121oC. Al-
low tubes to cool in a slanted position.

Use: For the cultivation and differentiation of mem-
bers of the Enterobacteriaceae based on their ability
to decarboxylate lysine and to form H2S. Bacteria
that decarboxylate lysine turn the medium purple.
Bacteria that produce H2S appear as black colonies.


Lysine Ornithine Mannitol Agar

(LOM Agar)

Composition per liter:

Agar .............................................................. ...13.5g

L-Ornithine*HCl ............................................... .6.5g

D-Mannitol ...................................................... .5.25g

L-Lysine*HCl................................................... ...5.0g

NaCl................................................................. ..5.0g

Yeast extract..................................................... ..3.0g

Bromthymol Blue ............................................ ..0.3g

Vancomycin solution................................... .10.0mL

pH 6.5 ± 0.2 at 25oC

Vancomycin Solution:

Composition per 10.0mL:

Vancomycin*HCl............................................ ..0.03g

Preparation of Vancomycin Solution: Add
vancomycin to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex-
cept vancomycin solution, to distilled/deionized wa-
ter and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure-121oC. Cool to 45o-50oC.
Aseptically add sterile vancomycin solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.

Use: For the cultivation and differentiation of Gram-
negative bacilli based on their ability to decarboxy-
late lysine or ornithine and mannitol fermentation. 

Especially useful for the identification of Entero-

bacter agglomerans. Bacteria that ferment mannitol
appear as dark yellow colonies. Bacteria that decar-
boxylate lysine or ornithine appear as green-yellow
colonies.