Liver Veal Egg Yolk Agar

Composition per 1080.0mL:

Liver veal agar ............................................... ... 1.0L

Egg yolk emulsion, 50%.............................. .80.0mL

pH 7.3 ± 0.2 at 25oC

Liver Veal Agar:

Composition per liter:

Veal, infusion from ...................................... ..500.0g

Beef liver, infusion from................................. ..50.0g

Gelatin........................................................... ...20.0g

Proteose peptone............................................. ..20.0g

Agar .............................................................. ...15.0g

Soluble starch.................................................. .10.0g

Glucose ........................................................... ...5.0g

NaCl................................................................. ..5.0g

Casein.............................................................. ...2.0g

NaNO3.............................................................. ..2.0g

Enzymatic digest of protein............................... ..1.3g

Pancreatic digest of casein................................. .1.3g

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Liver Veal Agar: Add compo-
nents to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Distribute into tubes or flasks. Make sure
that some liver and veal particles are transferred to
each tube. Autoclave for 15 min at 15 psi pressure-
121oC. Cool to 50oC.

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks............................................... ..11

Whole chicken egg................................................ ...1

NaCl (0.9% solution)................................. ...50.0mL

Preparation of Egg Yolk Emulsion, 50%:

Soak eggs with 1:100 dilution of saturated mercuric
chloride solution for 1 min. Crack eggs and separate
yolks from whites. Mix egg yolks with 1 chicken egg.
Beat to form emulsion. Measure 50.0mL of egg yolk
emulsion and add to 50.0mL of 0.9% NaCl solution.
Mix thoroughly. Filter sterilize. Warm to 45o-50oC.
Preparation of Medium: To 1.0L of cooled ster-
ile liver veal agar, aseptically add 80.0mL of sterile
egg yolk emulsion, 50%. Mix thoroughly. Pour into
sterile Petri dishes. Dry plates at 35oC for 24h.
Use: For the cultivation of a variety of anaerobic or-
ganisms.


LMX Broth Modified, Fluorocult
     (Fluorocult LMX Broth, Modified)
Composition per liter:

Tryptose ........................................................... ..5.0g

NaCl.................................................................. ..5.0g

Sorbitol ........................................................... ...1.0g

Tryptophan......................................................... .1.0g

K2HPO4........................................................... ...2.7g

KH2PO4........................................................... ...2.0g

Lauryl sulfate sodium salt................................. ..0.1g

5-Bromo-4-chloro-3-indolyl-β-D-

galactopyran_oside (X-GAL)..................... ..0.08

4-Methylumbelliferyl-β-D-glucuronide.......... ...0.05g

1-Isopropyl-β-D-1-thio-galactopyranoside
       IPTG) ... ....................................................... .0.1

pH: 6.8 ± 0.2 at 25oC

Source: This medium is available from Merck.
Preparation of Medium: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into into test tubes Autoclave for 15 min at 15 psi pressure-121oC. The broth is clear and yellowish brown.

Use:  For  the  simultaneous  detection  of  total coliforms and E. coli by the fluorogenic procedure. A color change of the broth from yellow to blue-green indicates the presence of coliforms. A blue fluores-
cence under long-wave UV light permits the rapid detection of E.coli. As tryptophan is added to the broth, the indole reaction is easily done by adding KOVACS reagent. The formation of a red ring addi-
tionally confirms the presence of E.coli.


Loeffler Blood Serum Medium

 Composition per liter:

Beef blood serum....................................... . 750.0mL

Dextrose broth ......................................... .. 250.0mL

pH 7.1 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Dextrose Broth:

Composition per liter:

Tryptose ........................................................ ...10.0g

Glucose ........................................................... ...5.0g

Sodium chloride................................................ ..5.0g

Beef extract...................................................... ...3.0g

Preparation of Dextrose Broth: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine 750.0mL of beef blood serum with 250.0mL of dextrose broth. Mix thoroughly. Distribute into screw-capped tubes. Slant tubes in the autoclave. Close the autoclave door loosely. Autoclave for 10 min at 0 psi pressure-
100oC. Close the autoclave door tightly. Autoclave for 15 min at 15 psi pressure-121oC.

Use: For the cultivation of Corynebacterium diph-
theriae. For demonstration of pigment production
and proteolysis by Corynebacterium diphtheriae.


Loeffler Blood Serum Medium

Composition per liter:

Beef blood serum....................................... .750.0mL

Dextrose broth......................................... ...250.0mL

pH 7.1 ± 0.2 at 25oC

Dextrose Broth:

Composition per liter:

Enzymatic digest of protein............................... ..2.5g

Glucose ........................................................... .1.25g

NaCl.............................................................. ...1.25g

Beef extract...................................................... .0.75g

Preparation of Dextrose Broth: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine 750.0mL of beef blood serum with 250.0mL of dextrose broth. Mix thoroughly. Distribute into screw-capped tubes. Slant tubes in the autoclave. Close the autoclave door loosely. Autoclave for 10 min at 0 psi pressure-
100oC. Close the autoclave door tightly. Autoclave for 15 min at 15 psi pressure-121oC.

Use: For the cultivation of Corynebacterium diph-
theriae. For demonstration of pigment production
and proteolysis by Corynebacterium diphtheriae.


Loeffler Medium

Composition per liter:

Beef serum...................................................... ..70.0g

Egg, dried........................................................ ...7.5g

Heart muscle, solids from infusion.................. .0.72g

Glucose ........................................................... .0.71g 

Peptic digest of animal tissue ........................ ...0.71g

NaCl............................................................... ...0.36g

pH 7.6 ± 0.2 at 25oC

Source: This medium is available as a premixed powder from  BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into screw-capped tubes. Slant tubes in the autoclave. Close the autoclave door loosely. Autoclave for 10 min at 0 psi pressure-
100oC. Close the autoclave door tightly. Autoclave for 15 min at 15 psi pressure-121oC.

Use: For the cultivation of Corynebacterium diph-
theriae. For demonstration of pigment production
and proteolysis by Corynebacterium diphtheriae. For
the cultivation and maintenance of Moraxella lacu-
nata.


Loeffler Slant

Composition per liter:

Tryptose ........................................................... ..5.0g

Glucose ........................................................... ...1.0g

Beef serum................................................ .. 750.0mL

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into screw-capped tubes. Slant tubes in the autoclave. Close the autoclave door loosely. Autoclave for 10 min at 0 psi pressure-
100oC. Close the autoclave door tightly. Autoclave for 15 min at 15 psi pressure-121oC.

Use: For the cultivation of Corynebacterium diph-
theriae. For demonstration of pigment production
and proteolysis by Corynebacterium diphtheriae. For
the cultivation and maintenance of Moraxella lacu-
nata.


Loeffler Slant, Modified

Composition per liter:

Glucose ........................................................... ...1.0g

Peptone ........................................................... ...0.5g

Beef serum................................................ .. 300.0mL

pH 7.6 ± 0.2 at 25oC

Preparation of Medium: Add peptone and glu-
cose to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Add beef serum. Mix thor-
oughly. Adjust pH to 7.6. Distribute into screw-capped
tubes in 3.0mL volumes. Slant tubes in the autoclave.
Autoclave for 30 min at 0 psi pressure-100oC.
Use: For the cultivation of Corynebacterium diphthe-
riae. For demonstration of pigment production and
proteolysis by Corynebacterium diphtheriae. For the
cultivation and maintenance of Moraxella lacunata.


Lombard-Dowell Agar

(LD Agar)

Agar .............................................................. ...20.0g

Pancreatic digest of casein................................. .5.0g

Yeast extract..................................................... ..5.0g

NaCl................................................................. ..2.5g

L-Cystine ........................................................... .0.4g

L-Tryptophan..................................................... .0.2g

Na2SO3.............................................................. .0.1g

Hemin........................................................... .10.0mg

NaOH (1N)................................................. ...5.0mL

Vitamin K1 solution....................................... ..1.0mL

pH 7.5 ± 0.2 at 25oC

Vitamin K1 Solution:

Composition per 100.0mL:

Vitamin K1 ........................................................ .1.0g

Ethanol......................................................... .99.0mL

Preparation of Vitamin K1 Solution: Add vita-
min K1 to 99.0mL of absolute ethanol. Mix thoroughly.

Preparation of Medium: Add hemin and L-cys-
tine to 5.0mL of NaOH. Mix thoroughly. Add re-
maining components. Bring volume to 1.0L with distilled/deionized water. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-
121oC. Pour into sterile Petri dishes.

Use: For the cultivation and identification of a variety of
obligate  anaerobic  bacteria.  For  the  cultivation  of

Bacteroides species, Fusobacterium species, Clostridium

species, and nonspore-forming Gram-positive anaerobes.


Lombard-Dowell Bile Agar

(LD Bile Agar)

Composition per liter:

Agar .............................................................. ...20.0g

Oxgall.............................................................. .20.0g

Pancreatic digest of casein................................. .5.0g

Yeast extract..................................................... ..5.0g

NaCl................................................................. ..2.5g

D-Glucose........................................................ ...1.0g

L-Cystine ........................................................... .0.4g

L-Tryptophan..................................................... .0.2g

Na2SO3.............................................................. .0.1g

Hemin........................................................... .10.0mg

NaOH (1N)................................................. ...5.0mL

Vitamin K1 solution ...................................... .1.0mL

pH 7.5 ± 0.2 at 25oC

Vitamin K1 Solution:

Composition per 100.0mL